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From: TSS ()
Subject: Re: Feds skipped key mad cow disease test in 2004 case USDA changes its protocols after animal initially had been cleared
Date: June 17, 2005 at 5:03 am PST

In Reply to: Feds skipped key mad cow disease test in 2004 case USDA changes its protocols after animal initially had been cleared posted by TSS on June 17, 2005 at 4:52 am:

-------- Original Message --------
Subject: RE: Greetings again Professor Aguzzi ... TSS
Date: Fri, 11 Mar 2005 09:19:49 +0100
From: "Adriano Aguzzi"
To: "'Terry S. Singeltary Sr.'"


Dear Mr. Singeltary

I sympathize with your wish to have the most sensitive assay implemented.
However, the situation is not as simple as one might think. In the case of
homogeneously distributed agent, biochemical detection of PrPSc is indeed
likely to be more sensitive than immunohistochemistry. In the case of
variegated, punctate distribution of the agent, morphological methods may
indeed be an asset.

There are also issues of feasibility. In my laboratory, we routinely run
phosphotungstic acid precipitation followed by Western blotting. However,
this is an extraordinarily cumbersome procedure. The sensitivity is
increased vastly, but the amount of work needed is also amazing. There is
no way I could see our own procedure implemented for mass screening of
millions of cows - unless one would draft a veritable army of laboratory
technicians.

For all these reasons, while I see all your points, I feel unable to offer a
strong public opinion in favor or against any specific methods. The final
decision needs to take into account a variety of complex factors, and that
is why I believe that it is best left to a panel of experts rather than to a
public discussion.

Best regards
Adriano Aguzzi
____________________________
Prof. Adriano Aguzzi
(MD PhD hc FRCP FRCPath)
Institute of Neuropathology, University Hospital of Zürich
Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
Tel. ++41-1-255 2107
Tel. (direct line): 2869
Fax: ++41-1-255 4402, cellular: +41-79-320 1516
http://www.unizh.ch/pathol/neuropathologie/

-----Original Message-----
From: Terry S. Singeltary Sr. [mailto:flounder@wt.net]
Sent: Thursday, March 10, 2005 20:18
To: adriano@pathol.unizh.ch
Subject: Greetings again Professor Aguzzi ... TSS

Greetings again Professor Aguzzi,

A kind greetings from Texas. I hope you do not mind, but I
must ask you several questions that will put you in the hot seat.
Someone with credibility must come forward, such as yourself
and speak out about the fact of the non scientific approach that
USDA et al has take after the first diagnosis of BSE in the USA.
This being, the refusal to use Western Blot on any suspicious or
inconclusive BSE/TSE test. IHC is like a brain biopsy on trying
to diagnose a CJD case. IF you take the sample from a part of the
brain that is not that tainted, you will not get a reading. WB is much
more sensitive, especially now with the Phospohtugstic acid
precipitation step. IF Prusiners CDI was validated, who knows,
that might even be more sensitive. Bottom line, we need you to
come forward and state publicly ''the facts'' about USDA et al
decision not to use WB on not only questionable samples, but on
ALL samples. would you be willing to comment on this, to me or
someone from the media (under the understanding it will be for
the public)? I have several questions for you??? This is very very
important in terms of human health (i.e. that nov. pos. pos. incl. neg
cow).

P.S. there is one other top TSE scientist that has come forward
and said what the USDA et al did with that cow was ''not logical''.
(this will not be published for another 3 or 4 weeks).
ONE other TOP TSE scientist saying the same thing would be
much better for the public to hear and understand. anyway,
does not hurt to ask, and i hope you come through here for us.
I know this is a very loaded question, but times a wasting, and
human health is at risk here...

thank you,
with kindest regards,

I am sincerely,

Terry S. Singeltary Sr.


CJD WATCH

http://www.fortunecity.com/healthclub/cpr/349/part1cjd.htm


CJD Watch message board

http://disc.server.com/Indices/167318.html






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