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From: TSS ()
Subject: Re: U.S. checking for possible case of mad cow disease Friday, June 10, 2005
Date: June 12, 2005 at 2:54 pm PST

In Reply to: Re: U.S. checking for possible case of mad cow disease Friday, June 10, 2005 posted by TSS on June 11, 2005 at 1:28 pm:

Release No. 0207.05
Contact:
USDA Press Office (202)720-4623


Transcript of Tele-News Conference with Agriculture Secretary Mike Johanns and Dr. John Clifford, Chief Veterinary Officer, Animal Plant Health Inspection Service Regarding further analysis of BSE Inconclusive Test Results Washington, D.C.
June 10, 2005

snip...

DR. CLIFFORD: "Thank you, Mr. Secretary.

snip...

>>>"In addition, there are definite differences between these two tests. The IHC is internationally recognized, and why we chose that for our enhanced surveillance program is because that particular test does two things. It allows you to visualize the anatomic location where the lesions are most likely to be found which is the obex. At the same time it uses a staining technique on the prions, on abnormal prions in the tissue in that location. ...<<<

http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1OB?contentidonly=true&contentid=2005/06/0207.xml


We
have to be careful that we donít get so set in the way we do things that we
forget to look for different emerging variations of disease. We've gotten
away from collecting the whole brain in our systems. We're using the brain
stem and we're looking in only one area. In Norway, they were doing a
project and looking at cases of Scrapie, and they found this where they did
not find lesions or PRP in the area of the obex. They found it in the
cerebellum and the cerebrum. It's a good lesson for us. Ames had to go
back and change the procedure for looking at Scrapie samples. In the USDA,
we had routinely looked at all the sections of the brain, and then we got
away from it. They've recently gone back.
Dr. Keller: Tissues are routinely tested, based on which tissue provides an
Ďofficialí test result as recognized by APHIS
.

Dr. Detwiler: That's on the slaughter. But on the clinical cases, arenít
they still asking for the brain? But even on the slaughter, they're looking
only at the brainstem. We may be missing certain things if we confine
ourselves to one area.

snip.............

Dr. Detwiler: It seems a good idea, but I'm not aware of it.
Another important thing to get across to the public is that the negatives
do not guarantee absence of infectivity. The animal could be early in the
disease and the incubation period. Even sample collection is so important.
If you're not collecting the right area of the brain in sheep, or if
collecting lymphoreticular tissue, and you don't get a good biopsy, you
could miss the area with the PRP in it and come up with a negative test.
There's a new, unusual form of Scrapie that's been detected in Norway. We
have to be careful that we donít get so set in the way we do things that we
forget to look for different emerging variations of disease. We've gotten
away from collecting the whole brain in our systems. We're using the brain
stem and we're looking in only one area. In Norway, they were doing a
project and looking at cases of Scrapie, and they found this where they did
not find lesions or PRP in the area of the obex. They found it in the
cerebellum and the cerebrum. It's a good lesson for us. Ames had to go
back and change the procedure for looking at Scrapie samples. In the USDA,
we had routinely looked at all the sections of the brain, and then we got
away from it. They've recently gone back.

Dr. Keller: Tissues are routinely tested, based on which tissue provides an
Ďofficialí test result as recognized by APHIS
.

Dr. Detwiler: That's on the slaughter. But on the clinical cases, arenít
they still asking for the brain? But even on the slaughter, they're looking
only at the brainstem. We may be missing certain things if we confine
ourselves to one area.

snip...

FULL TEXT;

Completely Edited Version
PRION ROUNDTABLE


Accomplished this day, Wednesday, December 11, 2003, Denver, Colorado


http://www.vegsource.com/talk/madcow/messages/94543.html

TSS




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