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From: TSS ()
Subject: SCRAPIE USA MONTHLY REPORT 2005 (what about those ATYPICAL VERMONT TSE SHEEP MOUSE BIO-ASSAYs?)
Date: May 30, 2005 at 2:48 pm PST

AS of March 31, 2005, there were 70 scrapie infected source flocks (Figure 3). There were 11 new infected and source flocks reported in March (Figure 4) with a total of 51 flocks reported for FY 2005 (Figure 5). The total infected and source flocks that have been released in FY 2005 are 39 (Figure 6), with 1 flock released in March. The ratio of infected and source flocks released to newly infected and source flocks for FY 2005 = 0.76 : 1. IN addition, as of March 31, 2005, 225 scrapie cases have been confirmed and reported by the National Veterinary Services Laboratories (NVSL), of which 53 were RSSS cases (Figure 7). This includes 57 newly confirmed cases in March 2005 (Figure 8). Fourteen cases of scrapie in goats have been reported since 1990 (Figure 9). The last goat cases was reported in January 2005. New infected flocks, source flocks, and flocks released or put on clean-up plans for FY 2005 are depicted in Figure 10. ...

FULL TEXT ;

http://www.aphis.usda.gov/vs/nahps/scrapie/monthly_report/monthly-report.html


NO REPORT OR UPDATE ON THOSE VERMONT SHEEP THAT A DECLARATION OF EMERGENCY WAS DECLARED DUE TO ATYPICAL TSE HERE IN USA ...TSS


Release No. 0141.02


#


Imported
Belgium/Netherlands
Sheep Test Results
Background
Factsheet
Veterinary Services April 2002
APHIS


snip...

Additional tests will be conducted to determine
exactly what TSE the animals haveBSE or scrapie.
These tests involve the use of bioassays that consist
of injecting mice with tissue from the infected animals
and waiting for them to develop disease. This testing
may take at least 2 to 3 years to complete.


http://www.aphis.usda.gov/lpa/pubs/fsheet_faq_notice/fs_ahvtsheeptr.pdf

DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E.
(PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES

http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci

d=fr20jy00-32


DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
(PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]

http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci

d=fr20jy00-31


or if those old urls dont work, go here;

DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
(PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
- Terry S.
Singeltary Sr. 7/20/00 (0)

http://www.vegsource.com/talk/madcow/messages/7507.html

I was told that ;


-------- Original Message --------
Subject: Re: hello Dr. Sutton...question please...scrapie...TSS
Date: Thu, 20 May 2004 14:36:09 -0400
From: Jim.D.Rogers@aphis.usda.gov
To: flounder@wt.net

Dear Mr. Singeltary,

The Western blot tests on these animals were completed in April of this
year. That means that we can begin the mouse inoculations. To get the
results of the Western blot tests, you will need to submit a Freedom of
Information Act request through our FOIA office. The FAX number there is
301-734-5941.

Have a nice day,

Jim Rogers
APHIS LPA
=========


-------- Original Message --------

Subject: Sheep
Date: Sat, 12 Jun 2004 14:26:04 EDT
From: LAVET22@aol.com
To: flounder@wt.net


Mr. Singeltary.

I hope this finds you well. As you are aware I left the USDA last year. I can only update you on the sheep before that time. Contact was established with the UK on doing the bioassay studies. They agreed. However, we were prioritized after their own needs, hence the delay. I am aware that there are now additional labs in Europe running the mouse bioassay strain typing. You will have to contact USDA for further word.


Linda Detwiler

==========


-------- Original Message -------- Subject: re-85th Meeting of SEAC - 30.11.04
Date: Tue, 21 Dec 2004 16:56:55 -0000
From: "Barlow, Tom (SEAC)"
To: "'flounder@wt.net'"


Dear Mr SingeltaryThank you for you enquiry to the SEAC secretariat about mouse bioassayscommissioned by the USDA to investigate TSE cases in imported sheep. After making a number of enquiries, it appears that Defra were not involvedwith this work. However, it is possible that a UK research laboratory wascontacted by the USDA about such tests but I have been unable to find outany further information. You may wish to make further enquiries with theUSDA.Yours sincerelyTom BarlowDr Tom BarlowSpongiform Encephalopathy Advisory Committee (SEAC) SecretariatArea 108, 1A Page Street, London SW1P 4PQTel: 0207 904 6267 -----Original Message----- From: Terry S. Singeltary Sr. [mailto:flounder@wt.net] Sent: 02 December 2004 20:19 To: Dale, Tabitha J (SEAC) Subject: re-85th Meeting of SEAC - 30.11.04 Hello Tabitha, A kind greetings from Texas. I had signed up for the meeting and wanted to ask aquestion, but it took me too long to finally get everything working properly on myend with the viewing. finally got things going today and got into theaudio of the meeting (will have to download an upgrade for my windowsmedia). ASIDE from the disturbing points made about sCJD not beingtied to BSE from some unpublished mouse bioassays (if i heardthat right) and the fact that they still today base the increase ofsporadic CJD in known BSE countries as a happenstance of bettersurveillance, I wish to kindly ask a question not pertaining to the above, asdisturbing as it is (lost my mother to the hvCJD 12/14/97) and i simplyhave never accepted the spontaneous/sporadic aspects of this agent in85%+ of all humans. never will, it's a pipe dream thought up in someback room in the 80s to protect the industries involved (my opinion). MY question is one about the VERMONT USA SHEEP that were imported to the USA from Belgium and confiscated by the USDAseveral years ago due to an atypical TSE, with the announcement thatmouse studies would be immediately started. I was informed by Dr.Linda Detwiler that it was DEFRA that was responsible for these mouse studies beingput on hold for 2 years. WHY were such important studies put off for 2years by DEFRA? HERE is my correspondence with Dr. Detwiler; Release No. 0141.02 Ed Curlett (301) 734-3256 Jerry Redding (202) 720-6959 TESTING TO CONTINUE ON IMPORTED SHEEP CONFISCATED LAST YEAR WASHINGTON, April 11, 2002 -- The U.S. Department ofAgriculture today announced that tests conducted on a flock of sheepconfiscated last year from a farm in Vermont confirm that two of the 125 sheeptested positive for an atypical undifferentiated transmissible spongiformencephalopathy (TSE) of foreign origin. The flock of 125 sheep wasconfiscated in March 2001 after four animals from an associated flock testedpositive for TSE in July 2000. USDA will continue to conduct additional teststo determine the type of TSE in these sheep. "These results confirm our previous conclusions were correctand that we took the appropriate preventative actions in confiscatingthese animals," said Bobby Acord, administrator of USDAs Animaland Plant Health Inspection Service. "USDAs actions to confiscate,sample and destroy these sheep were on target. As a result of ourvigilance, none of these confiscated animals entered the animal or humanfood supply." The sheep, imported from Belgium and the Netherlands in1996, were placed under certain federal restrictions when they enteredthe country as part of USDA's scrapie control efforts. In 1998, USDAlearned that it was likely that sheep from Europe were exposed to feedcontaminated with bovine spongiform encephalopathy. At that time, the state ofVermont, at the request of USDA, imposed a quarantine on these flocks,which prohibited slaughter or sale for breeding purposes. On July 10, 2000, several sheep from the flock testedpositive for a TSE, a class of degenerative neurological diseases that ischaracterized by a very long incubation period and a 100 percent mortalityrate in infected sheep. Two of the better known varieties of TSE arescrapie in sheep and BSE in cattle. There is no evidence that scrapieposes a risk to human health. On July 14, 2000, USDA issued a declaration of extraordinaryemergency to acquire the sheep. This action was contested by the flockowners. A federal district court judge ruled in favor of USDA based onthe merits of the case. The flock owners appealed to the Second CircuitCourt requesting a stay, which was denied. The sheep wereconfiscated by USDA in March 2001 and transported to USDA's National VeterinaryServices Laboratories in Ames, Iowa, where they were humanelyeuthanized. Tissue samples were collected from the sheep for diagnostic testingand USDA will continue with additional tests which could take up to 2- 3 years to complete. In all, USDA has acquired 380 sheep from atotal of three flocks. All of the animals were humanely euthanized, sampledand disposed and did not enter the animal or human food supply. Our goal continues to be to prevent, detect and eradicateforeign animal diseases to protect American agriculture, natural resourcesand consumers," said Acord. "We will continue to utilize thescientific results of these and other tests conducted during the lastseveral years to strengthen our extensive surveillance, monitoring andprevention efforts." For more information about USDAs ongoing surveillance,monitoring and prevention efforts as it relates to this situation, pleasevisit www.aphis.usda.gov/oa/tse/index.html # NOW, June 2004 those same test that we were told would startin 2002, have yet to be started. THE TSE those VERMONT sheep was supposedly to have had, has yet to be confirmed. WHY? Correspondence from Dr. Detwiler to me; -------- Original Message -------- Subject: Sheep Date: Sat, 12 Jun 2004 14:26:04 EDT From: LAVET22@aol.com To: flounder@wt.net Mr. Singeltary. I hope this finds you well. As you are aware I left theUSDA last year. I can only update you on the sheep before that time.Contact was established with the UK on doing the bioassay studies. Theyagreed. However, we were prioritized after their own needs, hencethe delay. I am aware that there are now additional labs in Europerunning the mouse bioassay strain typing. You will have to contact USDA forfurther word. Linda Detwiler ========= >However, we were prioritized after their own needs, hencethe delay. > IF this was the case, this is totally unacceptable. FORsomething that has been ongoing since the 80s (BSE in SHEEP/GOATS) yet stillunresolved, there is absolutely no excuse why these studies were put off. withthe other sheep brain mix-up and now the BSE in the French Goat, I find itvery disturbing that the Vermont Sheep studies were put off for 2 years forwhatever reason, especially with the findings Dormont*, and Jean-PhilippeDeslys* et al, that The agent responsible for French iatrogenic growthhormone-linked CJD taken as a control is very different from vCJD but issimilar to that found in one case of sporadic CJD and one sheep scrapieisolate; http://www.pnas.org/cgi/content/full/041490898v1 YES, i am still very angry, but i want to still thank SEACfor the work they have done, i only wish things would go much faster andthat the BSE/nvCJD only theory would be put to rest once and for all.Science has pretty much proven that it was a pipe dream, howeverscience does not have near as much to do with this mess anymore asthe industry and politics do. it's simply not about scienceanymore. IN the USA, you dont even hear of these new studies from the Gov. and very little from the media... thank you, kindest regards, Terry S. Singeltary Sr. P.O. Box 42 Bacliff, TEXAS USA 77518The original of this email was scanned for viruses by the Government Secure Intranet (GSi) virus scanning service supplied exclusively by Energis in partnership with MessageLabs.On leaving the GSi this email was certified virus-free
TSS SUBMISSION TO VERMONT HEALTH DEPT. ; Health Alert on Certain Sheep Milk Cheese 2000 TSS SUBMISSION VERMONT HEALTH 2005-------------------------------------------------------------------------------- Original Message --------Subject: CJD HUMAN/ANIMAL TSEs VERMONT i.e. ATYPICAL VERMONT SHEEP FROMBELGIUM AND THAT CHEESE/MILKDate: Sat, 05 Feb 2005 21:22:59 -0600From: "Terry S. Singeltary Sr.To: bapao@vdh.state.vt.us CC: lcrist@vdh.state.vt.us , smoffat@vdh.state.vt.us Greetings to Vermont Department of Health Division of Health Surveillance P.O. Box 70 Burlington, VT 05402-0070 Agency of Human Services Jan K. Carney, MD, MPH Commissioner Ann R. Fingar, MD, MPH State Epidemiologist Managing Editor Apao,William K Moffatt, Sharon G I would like to write to you about my concerns of the mostrecent findings of BSE to the GOAT under natural fieldconditions and the recent findings of the Atypical Case of BovineSpongiform Encephalopathy in an East-Flemish Cow in Belgium,in relations to the ATYPICAL VERMONT SHEEP and thecheese and milk that was distributed to the public. THIS shouldhighten the concern of that situation, that was in all essence,given the ALL CLEAR. I would also like to know why theUSDA put off those mouse bio assays of those sheep inVermont for 2 years, when we were told they had started? July 19, 2000 Health Alert on Certain Sheep Milk Cheese Based on advice from the Centers for Disease Control & Prevention, theVermont Health Department recommends that people not eat two brands ofVermont sheep milk cheese. (November 9, 2001: This Health Alert has been lifted.) Retrieved on 2/5/05, NO link to original warning...TSS http://www.healthyvermonters.info/news.shtml Vermont Department of HealthHealth Surveillance Division Reportable Diseases Updated 03/15/2004 Creutzfeldt-Jakob disease/transmissiblespongiform encephalopathies snip... Reporting of DiseasesThe law requires that health care providers report diseases of publichealth importance. Persons who are required to report: health carefacilities,health care providers, health maintenance organizations, hospitaladministrators, laboratory directors, managed care organizations, nursepractitioners, nurses, physician assistants, physicians, school healthofficials, town health officers. Cases of reportable diseases should bereported to the Division within 24 hours.24 Hour Telephone Reporting Line (802)951-4080 or 1-888-588-7781Consultation and Inquiries 802-863-7240 (7:45AM  4:30PM M-F) or1-800-640-4374 (VT only)Emergency Consultation after normal business hours also available atnumbers above http://www.healthyvermonters.info/hs/epi/idepi/reportable/CommunicableDiseaseRegs2004.pdf 1. Reportable Diseases and Syndromes (to include any rare infectiousdisease or one dangerous to public health)Any unexpected pattern of cases, suspected cases, deaths or increasedincidence of any other illness of majorpublic health concern, because of the severity of illness or potentialfor epidemic spread, which may indicate a newlyrecognized infectious agent, an outbreak, epidemic, related publichealth hazard or act of bioterrorism. snip... Creutzfeldt-Jakob disease/transmissiblespongiform encephalopathies snip... http://www.healthyvermonters.info/hs/epi/idepi/reportable/CommunicableDiseaseRegs2004.pdf FULL TEXT FOLLOW THREAD http://disc.server.com/discussion.cgi?disc=167318;article=1926;title=CJD%20WATCH TSS http://www.prwatch.org/forum/showthread.php?p=12566#post12566 BASEd on all this and THE FINDINGS OF ATYIPCAL TSE IN BELGIUM COW,makes you wonder just what those Vermont Belgium sheep really had just what tissuesare infectious to humans; Atypical Case of Bovine Spongiform Encephalopathy in an East-FlemishCow in Belgium H. De Bosschere, DVM, PhD S. Roels, DVM, PhD E. Vanopdenbosch, DVM, Lic Veterinary and Agrochemical Research Centre (CODA/CERVA) National Reference Laboratorium for Veterinary TSEs Groeselenberg 99, B-1180 Ukkel (Brussels), Belgium KEY WORDS: Bovine spongiform encephalopathy, BSE, Western blot, atypicalBSE. ABSTRACT For many years, researchers believed that only one bovine spongiformencephalopathy (BSE) strain existed, in contrast to the many differentscrapie strains found. However, only very recently reports emerged aboutunconventional BSE strains seen in Italy, France, and Japan. The presentcase describes an atypical strain of BSE in Belgium in a 64-month-oldEast-Flemish cow with an electrophoretic profile and other featuressimilar to those described in Japan. INTRODUCTION snip... DISCUSSION For many years, researchers assumed that only one BSE strainexisted.710 Only in the past months, reports of atypical BSE caseswere announced.1113 The Japanese case11 describes a very young bull (23months) characterized by the absence of spongiform changes and PrPscdeposits immunohistochemically. The WB analysis revealed anelectrophoretic profile different from that of typical BSE,characterized by low content of the di-glycosylated molecular form ofPrPsc and a faster migration of the nonglycosylated form of PrPsc. InItaly,12 two BSE affected cattle with a previously unrecognizedneuropathologic profile and PrPsc type were seen. These cases weredetermined using a different staining pattern on immunohistochemistry, adifference in size and glycoform ratio of PrPsc on immunoblot and adifference in regional distribution of lesions. The two cases inFrance13 showed variant molecular features with a different PrPscelectrophoretic profile from other BSE cases, mainly characterized by ahigher molecular mass of the nonglycosylated PrPsc. The present caseshows the most similarities (ie, identical electrophoretic profile, onlyELISA and WB positive and histopathology and immunohistochemistrynegative) with the Japanese case,11 although the cow in the Japanesecase was only 23 months old, and the cow in this case was 64 months old. The fact that these strains were detected worldwide and in severalbreeds suggest that there is no local or breed-dependent featureinvolved. It could be that the WB techniques have become more specificwithin the past year in the detection of minor differences in di-,mono-, and nonglycosylated molecular forms of PrPsc. Infection of cattleby scrapie could also be considered since scrapie can be transmitted bydirect contact between animals or through environmental contamination.13 In conclusion, this Belgian case should be added to the list of atypicalBSE strains only very recently detected worldwide and may contribute tofurther research studies about epidemiologic significance. Currentcontinued research on BSE would appear to reveal different BSE strainsin analogy with the different scrapie strains. ACKNOWLEDGMENTS snip... http://www.jarvm.com/articles/Vol2Iss1/DEBOSSCHERE.htm NOT TO FORGET THIS FOR SURE; BASE in cattle in Italy of Identification of a second bovine amyloidotic spongiformencephalopathy: Molecular similarities with sporadic Creutzfeldt-Jakob disease http://www.pnas.org/cgi/content/abstract/0305777101v1 Adaptation of the bovine spongiform encephalopathy agent to primatesand comparison with Creutzfeldt- Jakob disease:Implications for human health THE findings from Corinne Ida Lasmézas*, [dagger] , Jean-Guy Fournier*,Virginie Nouvel*, Hermann Boe*, Domíníque Marcé*, François Lamoury*,Nicolas Kopp [Dagger ] , Jean-Jacques Hauw§, James Ironside¶, Moira Bruce [||] ,Dominique Dormont*, and Jean-Philippe Deslys* et al, that The agent responsible forFrench iatrogenic growth hormone-linked CJD taken as a control is very differentfrom vCJD but is similar to that found in one case of sporadic CJD and one sheep scrapie isolate; http://www.pnas.org/cgi/content/full/041490898v1 Characterization of two distinct prion strains derived from bovine spongiform encephalopathytransmissions to inbred mice http://vir.sgmjournals.org/cgi/content/abstract/85/8/2471 THE findings from the cow in Japan with tissue infectivity in theperipheral nerve tissue, suprarenal gland,First time from non-Specified Risk Material ; Japan Consumer Press onlineNippon shouhisha shinbunhttp://www.jc-press.com/eg.htmhttp://www.jc-press.com/En/Latest%20News/200411/20041109BSE%20death%20cow%27s%20anomalous%20prion.htmLast modified, 11/09/2004 13:42:49BSE death cow's anomalous prion detected from peripheral nerve tissue,suprarenal glandFirst time from non-Specified Risk Material, or SRMBy JCPRESSNational Institute of Animal Health Animal announced on November 1 that it had detected the anomalous prion protein that was the etiologic agent of the mad cow disease, or BSE, or bovine spongiform encephaalopathy, from the peripheral nerve tissue and the suprarenal gland of the cow of the age in the mad cow disease for the dying infection 94 months on March 9 this year.Japan is obligating the removal of the Specified Risk Material, or SRM such as the head, the spinal cord, the vertebral columns, and the smallintestines that accumulate the anomalous prion protein easily as a BSE(bovine spongiform encephaalopathy) measures.Because the mad cow disease etiologic agent was detected from a tissuedifferent from the Specified Risk Material, or SRM, the review of theSpecified Risk Material, or SRM might be urged on the Japanese Government.International Symposium of PRION DISEASES for food and drug safetyhttp://www.knt.co.jp/ec/2004/prion/national institute of animal health(only in Japanese)http://niah.naro.affrc.go.jp/index-j.htmlThe statement of the Ministry of Health, Labour and Welfare(only in Japanese)http://www.maff.go.jp/www/press/cont2/20041101press_7.htmYomiuri on line (only in Japanese)http://www.yomiuri.co.jp/science/news/20041102i503.htmAsahi on line(only in Japanese)http://www.asahi.com/special/bse/TKY200411010291.htmlMainichi on line(only in Japanese)http://www.mainichi-msn.co.jp/shakai/jiken/disease/news/20041102ddm041040128000c.html WITH all this TSE SCIENCE, i would just sit around and flounder another 2-3 yearson those mouse bio assays on those Vermont sheep, deny that any of it ever happened,and by all means implement GWs Minimal Risk Region for TSEs (MRR) or what i callthe Legal Trading of TSEs (MRRLTTSE) POLICY for short, flounder a little longer,continue to use the SSS policy of Canada's on any other mad cows in Texas or any whereelse, then just pass the buck off to the next administration. problem solved. been going onfor decades... still disgusted in Bacliff, Texas USA...TSS





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