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From: TSS ()
Subject: Sensitive Detection of Prion Protein in Human Urine
Date: May 28, 2005 at 11:17 am PST

##################### Bovine Spongiform Encephalopathy #####################

Experimental Biology and Medicine 230:343-349 (2005)
© 2005 Society for Experimental Biology and Medicine


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ORIGINAL RESEARCH ARTICLE
Sensitive Detection of Prion Protein in Human Urine
Harash K. Narang*,2, Ayuna Dagdanova, Zhiliang Xie, Qiwei Yang and Shu G.
Chen,1
* BioTech Global, 22-40 Brentwood Avenue, Newcastle Upon Tyne, NE2 3DH, UK;
and Institute of Pathology, Case Western Reserve University, 2085 Adelbert
Road, Cleveland, Ohio 44106

To whom requests for reprints should be addressed at 1 Institute of
Pathology, Case Western Reserve University, 2085 Adelbert Road, Cleveland,
OH 44106. E-mail: shu.chen@case.edu

Transmissible spongiform encephalopathies are a group of infectious diseases
typically associated with the accumulation of a protease-resistant and
ß-sheet-rich prion protein, PrPSc, in affected brains. PrPSc is an altered
isoform derived from the host-encoded glycoprotein, PrPC. The expression of
PrPC is the highest in brain tissue, but it can also be detected at low
levels in peripheral tissue. However, it is unclear whether a significant
amount of PrPC is released into body fluid and excreted into urine. We have
developed a simple, rapid method for the reliable detection of PrPC in urine
from normal subjects by Western blotting. Our method can easily and reliably
detect PrPC in apparently healthy individuals using less than 1 ml of urine
in which the amount of urinary PrPC is estimated to be in the range of low
micrograms/liter.


Key Words: urine . prion protein . transmissible spongiform
encephalopathies . bovine spongiform encephalopathy . Creutzfeldt-Jakob
disease

http://www.ebmonline.org/cgi/content/abstract/230/5/343

TSS

----- Original Message -----
From: "Terry S. Singeltary Sr."
To:
Sent: Saturday, May 28, 2005 6:52 AM
Subject: Sensitive Detection of Prion Protein in Human Urine

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