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From: TSS ()
Subject: Immunohistochemical studies of scrapie archival material from Irish ARQ/ARQ sheep for evidence of BSE-derived disease
Date: May 18, 2005 at 6:08 pm PST

-------- Original Message --------
Subject: Immunohistochemical studies of scrapie archival material from Irish ARQ/ARQ sheep for evidence of BSE-derived disease
Date: Tue, 17 May 2005 17:10:28 -0500
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@aegee.org


##################### Bovine Spongiform Encephalopathy #####################

1: Res Vet Sci. 2005 Aug;79(1):29-35. Epub 2004 Dec 16.


Immunohistochemical studies of scrapie archival material from Irish
ARQ/ARQ sheep for evidence of bovine spongiform
encephalopathy-derived disease.

Sharpe A, McElroy M, Langeveld JP, Bassett H, O Donoghue AM, Sweeney T.

Central Veterinary Research Laboratory (CVRL), Abbotstown,
Castleknock, Dublin 15, Ireland.

Since scrapie and bovine spongiform encephalopathy (BSE) in sheep
are clinicopathologically indistinguishable, BSE in sheep may have
been misdiagnosed as scrapie. Disease-specific prion protein
(PrP(d)) patterns in archival tissues of 38 Irish ARQ/ARQ sheep
diagnosed as scrapie-affected were compared to those in four Dutch
BSE-challenged sheep. When medulla oblongata was immunolabelled with
an antibody directed against amino acids 93-99 of ovine prion
protein (ovPrP), intraneuronal PrP(d) was apparent in all 38 Irish
sheep but was absent in BSE-challenged sheep. When lymphoid
follicles were immunolabelled with antibodies directed against amino
acids 93-106 of ovPrP, granule clusters of PrP(d) were seen in 34 of
the 38 Irish sheep. Follicles of the remaining four archive sheep
contained either no PrP(d) or single PrP(d) granules, similar to
follicles from BSE-challenged sheep. Based on the medulla results,
none of the archival cases had BSE-derived disease. The
identification of some scrapie sheep with little or no
intrafollicular PrP(d) suggests that this technique may be limited
in discriminating between the two diseases.

PMID: 15894021 [PubMed - in process]

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15894021

TSS

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