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From: TSS ()
Subject: Re: Research Project: Study of Atypical Bse Project Number: 3625-32000-073-07 ATYPICAL BSE/TSE
Date: May 7, 2005 at 5:21 pm PST

-------- Original Message --------
Subject: Re: Research Project: Study of Atypical Bse Project Number: 3625-32000-073-07
Date: Sun, 2 Jan 2005 11:21:30 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@LISTSERV.KALIV.UNI-KARLSRUHE.DE
References: <41D723D7.60809@wt.net>


##################### Bovine Spongiform Encephalopathy #####################

Greetings list members,

I was going over the data from the 1st documented BSE/TSE cow
in the USA and find it disturbing they thought it important enough
to use WB to verify there immunohistochemistry test then. HOWEVER,
on the 3, 4, and 5 mad cow in the USA, they refused to use WB
to confirm this. I guess it worked so well the first time they could not
afford to use it again. (please note the 2nd USA mad cow was the
one in TEXAS they cover-up after witnessing the stumbling and staggering
cow and then refusing to BSE/TSE test it, just decided to send to
the render to get rid of all evidence. SO, if you count that Texas cow,
there would have most likely have been 5 confirmed cases of BSE/TSE in
the USA, if they would have used the WB like they did on the first cow)...


TSEs Touch Off
ARS Research


A year ago this month, a group of ARS
scientists and technicians gave up their Christmas time off and even
delayed family vacations to provide characterization of the first case
of bovine spongiform encephalopathy (BSE)—commonly called mad cow
disease—to be found in the United States.

On December 23, 2003, a Canadian cow shipped to slaughter from a farm in
Mabton, Washington, had come up presumptively positive for BSE in
testing by USDA's Animal and Plant Health Inspection Service (APHIS),
which has diagnostic responsibility and regulatory oversight for BSE
issues. APHIS had already used the "gold standard" diagnostic
immunohistochemistry test, which was originally developed by ARS. But
for the first U.S. case of BSE, APHIS wanted additional scientific
information that could be provided by the Western blot test.

So APHIS put in a high-priority call to veterinary medical officer
Juergen Richt and his colleagues at the Virus and Prion Diseases of
Livestock Laboratory, which is part of ARS's National Animal Disease
Center (NADC) in Ames, Iowa.

"We had experience with the Western blot test and we had all the
reagents on hand," explains Richt. "So we put our holiday plans on hold
and got everything ready so that APHIS would have verification of the
results from the immunohistochemistry test." ........... snip

full text;

http://www.ars.usda.gov/is/AR/archive/dec04/tse1204.htm
http://www.ars.usda.gov/is/AR/archive/dec04/

TSS

Terry S. Singeltary Sr. wrote:

> ##################### Bovine Spongiform Encephalopathy
> #####################
>
> Research Project: Study of Atypical Bse
>
> Location:
>
>
>
> Virus and Prion Diseases of Livestock
>
>
> Project Number: 3625-32000-073-07
> Project Type: Specific C/A
>
> Start Date: Sep 15, 2004
> End Date: Sep 14, 2007
>
> Objective:
> The objective of this cooperative research project with Dr. Maria
> Caramelli from the Italian BSE Reference Laboratory in Turin, Italy,
> is to conduct comparative studies with the U.S. bovine spongiform
> encephalopathy (BSE) isolate and the atypical BSE isolates identified
> in Italy. The studies will cover the following areas: 1. Evaluation of
> present diagnostics tools used in the U.S. for the detection of
> atypical BSE cases. 2. Molecular comparison of the U.S. BSE isolate
> and other typical BSE isolates with atypical BSE cases. 3. Studies on
> transmissibility and tissue distribution of atypical BSE isolates in
> cattle and other species.
>
> Approach:
> This project will be done as a Specific Cooperative Agreement with the
> Italian BSE Reference Laboratory, Istituto Zooprofilattico
> Sperimentale del Piemonte, in Turin, Italy. It is essential for the
> U.S. BSE surveillance program to analyze the effectiveness of the U.S
> diagnostic tools for detection of atypical cases of BSE. Molecular
> comparisons of the U.S. BSE isolate with atypical BSE isolates will
> provide further characterization of the U.S. BSE isolate. Transmission
> studies are already underway using brain homogenates from atypical BSE
> cases into mice, cattle and sheep. It will be critical to see whether
> the atypical BSE isolates behave similarly to typical BSE isolates in
> terms of transmissibility and disease pathogenesis. If transmission
> occurs, tissue distribution comparisons will be made between cattle
> infected with the atypical BSE isolate and the U.S. BSE isolate.
> Differences in tissue distribution could require new regulations
> regarding specific risk material (SRM) removal.
>
> http://www.ars.usda.gov/research/projects/projects.htm?ACCN_NO=408490
>
>> Differences in tissue distribution could require new regulations
>> regarding specific risk material (SRM) removal.
>
>
>
>

snip...end

full text ;

http://www.bseinquiry.gov.uk/files/mb/m11b/tab01.pdf

It was, however, performed in the USA in 1979, when it was shown that cattle inoculated with the scrapie agent endemic in the flock of Suffolk sheep at the United States Department of Agriculture in Mission, Texas, developed a TSE quite unlike BSE. 32 The findings of the initial transmission, though not of the clinical or neurohistological examination, were communicated in October 1988 to Dr Watson, Director of the CVL, following a visit by Dr Wrathall, one of the project leaders in the Pathology Department of the CVL, to the United States Department of Agriculture. 33 The results were not published at this point, since the attempted transmission to mice from the experimental cow brain had been inconclusive. The results of the clinical and histological differences between scrapie-affected sheep and cattle were published in 1995. Similar studies in which cattle were inoculated intracerebrally with scrapie inocula derived from a number of scrapie-affected sheep of different breeds and from different States, were carried out at the US National Animal Disease Centre. 34 The results, published in 1994, showed that this source of scrapie agent, though pathogenic for cattle, did not produce the same clinical signs of brain lesions characteristic of BSE.

http://www.bseinquiry.gov.uk/report/volume2/chaptea3.htm

Visit to USA ... info on BSE and Scrapie

http://www.bseinquiry.gov.uk/files/yb/1988/10/00001001.pdf

WHY is USA insisting _now_ not to use WB, when on the 1st _confirmed_ case Dec. 23, 2003
USA mad cow, WB was used ???

maybe this is the reason ;

JAPAN BSE # 8 & 9 cow

8. 6/10/2003 Holstein Steer 13/10/2001 23 mths
No clinical signs WB+, IHC-, HP-


9. 4/11/2003 Holstein Steer 13/1/2002
21 mths No clinical signs WB+, IHC-, HP-

===========

More information on the first 11 Japanese BSE-cases can be found on the website of the Japanese Embassy in the US:

http://www.us.emb-japan.go.jp/english/html/fafacts/bse/bse.htm

it's gonna be a long year........

TSS

######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########




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