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##################### Bovine Spongiform Encephalopathy #####################
Jpn. J. Infect. Dis., 58, 78-82, 2005
Original Article
Infection Route-Independent Accumulation of Splenic Abnormal Prion Protein
Yuji Inoue1,2, Yoshio Yamakawa2, Akikazu Sakudo1, Tomoya Kinumi2, Yuko
Nakamura1,2, Yosh!tsugu Matsumoto1, Keiichi Saeki1, Tsuneo Kamiyama3,
Takashi Onodera1,4* and Masahiro Nishijima2
1Department of Molecular Immunology, School of Agricultural and Life
Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
2Department of Biochemistry and Cell Biology, and 3Department of
Veterinary Science, National Institute of Infectious Diseases, Tokyo
162-8640, Japan
(Received August 2, 2004. Accepted January 11, 2005)
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*Corresponding author: Mailing address: Department of Molecular
Immunology, School of Agricultural and Life Sciences, University of
Tokyo, Bunkyo-Ku, Tokyo 113-8657, Japan. Tel: +81-3-5841-5196, Fax:
+81-3-5841-8020, E-mail: aonoder@mail.ecc.u-tokyo.ac.jp
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SUMMARY: The accumulation kinetics of the abnormal form of prion protein
(PrPSc) in spleens and brains of scrapie (Obihiro-1)-infected mice at
various times after intracerebral (i.c.), intraperitoneal (i.p.), or
oral inoculation were studied. PrPSc was first detected by Western
blotting with anti-prion protein antibodies on days 70 and 116 after
i.c. (3 mg) in spleens and brains, respectively. Although the amount of
cerebral PrPSc gradually increased to the maximum level on day 152 after
i.c. inoculation, splenic PrPSc established the maximum level on day 116
after i.c. inoculation then registered slight decreases up to day 152
with further incubation. The detectable levels of cerebral PrPSc by
Western blotting were established on day 231 or 259, whereas those of
splenic PrPSc were detected on day 94 or 93, after i.p. and oral
infection, respectively. The splenic PrPSc decreased slightly
thereafter. These results indicate that splenic PrPSc increased before
cerebral PrPSc established a detectable level in a manner independent of
the inoculation route.
http://www.nih.go.jp/JJID/58/78.html
SEE FULL TEXT ;
http://www.nih.go.jp/JJID/58/78.pdf
P.S. I had to download into file first, then open, before pdf
file would work for me...
TSS
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