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From: TSS ()
Subject: Expression of PrPC on cellular components of sheep blood
Date: April 14, 2005 at 6:56 pm PST

-------- Original Message --------
Subject: Expression of PrPC on cellular components of sheep blood
Date: Thu, 14 Apr 2005 18:06:34 -0500
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@KALIV.UNI-KARLSRUHE.DE


##################### Bovine Spongiform Encephalopathy #####################

Expression of PrPC on cellular components of sheep blood

S. Halliday, F. Houston and N. Hunter

Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK

Correspondence
F. Houston
fiona.houston@bbsrc.ac.uk

PrPC, a glycosylphosphatidylinositol-linked glycoprotein, plays a
central role in the pathogenesis of transmissible spongiform
encephalopathies (TSEs), undergoing a conformational alteration to the
disease-associated isoform, commonly designated PrPSc. PrPC is expressed
in many tissues other than the nervous system, although its precise
function(s) remains unclear. It has previously been demonstrated that
TSEs can be transmitted by blood transfusion in sheep. The aim of this
work was to identify which components of blood carried the infection. As
an initial step, the distribution of PrPC on cellular components of
sheep blood was examined to identify potential targets for infection.
Cell-surface expression of PrPC was found only on peripheral blood
mononuclear cells (PBMCs); however, platelets also contained significant
amounts of intracellular PrPC. The level of PrPC expressed on the cell
surface of PBMCs was influenced by PrP genotype, with the highest levels
found in scrapie-susceptible VRQ/VRQ sheep and the lowest levels in
scrapie-resistant ARR/ARR sheep. In susceptible sheep, PrPC was
expressed at varying levels on all major subsets of PBMCs, with the
highest levels on the CD21+ subset of B cells, and PrP expression was
upregulated dramatically on CD21+ B cells in some scrapie-infected sheep.


http://vir.sgmjournals.org/cgi/content/abstract/86/5/1571?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=PRION&searchid=1113519731411_1431&stored_search=&FIRSTINDEX=0&volume=86&issue=5&search_url=http%3A%2F%2Fvir.sgmjournals.org%2Fcgi%2Fsearch

TSS

######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########






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