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From: TSS ()
Subject: Approaches to Scrapie diagnosis by applying immunohistochemistry and rapid tests on central nervous and lymphoreticular systems
Date: April 6, 2005 at 2:36 pm PST

Approaches to Scrapie diagnosis by applying immunohistochemistry and
rapid tests on central nervous and lymphoreticular systems

E. Monleón, M. MonzónCorresponding Author Contact Information , E-mail The Corresponding Author , P. Hortells, R. Bolea, C. Acín, F. Vargas and J.J. Badiola

National Reference Centre for Transmissible Spongiform Encephalopathies, University of Zaragoza, Avda. Miguel Servet 177, 50013 Zaragoza, Spain

Received 20 September 2004; revised 16 December 2004; accepted 8 January 2005. Available online 19 February 2005.


Abstract

Comparative studies evaluating the performance of Transmissible Spongiform Encephalopathies (TSE) rapid tests (validated for Bovine Spongiform Encephalopathy samples) on Scrapie samples have not been reported widely, particularly those dealing with lymphoreticular system tissues to a much lesser extent. The main objective of this study was to compare the ability of two current rapid tests (Western blot and Luminiscence Immunoassay Prionics®-Check; WB and LIA, respectively) to detect PrPsc using central nervous system as well as lymphoreticular system samples corresponding to naturally infected animals. Thirty-four Scrapie-affected sheep, 26 with clinical signs of the disease, were included in the study. Tonsil, retropharyngeal lymph node and medulla oblongata were assessed by three tests: immunohistochemistry (confirmatory test), WB and LIA (rapid tests). The conclusion which can be drawn from this study is the fact that all animals involved in the study, including those at a preclinical stage, could be diagnosed regardless of the test used (with immunohistochemistry consistently showing higher sensitivity) only when the analyses of both the central nervous system and the lymphoreticular system were considered. The choice of these tissues for routine diagnosis is, therefore, proposed as a valuable tool to highly reduce the number of undetected positive cases.

Keywords: Scrapie; Central nervous system; Lymphoreticular system; Rapid test; Immunohistochemistry

Corresponding Author Contact Information Corresponding author. Tel.: +34 976 762835; fax: +34 976 761608.

TSS



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