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From: TSS ()
Subject: Surveillance of Chronic Wasting Disease in Sika Deer, Cervus nippon, from Tokachi District in Hokkaido
Date: April 5, 2005 at 8:47 am PST

Surveillance of Chronic Wasting Disease in Sika Deer, Cervus nippon, from Tokachi District in Hokkaido

Natsumi KATAOKA1), Masakazu NISHIMURA2), Motohiro HORIUCHI3) and Naotaka ISHIGURO4)

1) Laboratory of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine
2) Laboratory of Pharmacology, Obihiro University of Agriculture and Veterinary Medicine
3) Laboratory of Prion Disease, Graduate School of Veterinary Medicine, Hokkaido University
4) Laboratory of Food and Environmental Hygiene, Faculty of Applied Biological Sciences, Gifu University

(Received 3-Sep-2004)
(Accepted 24-Nov-2004)

ABSTRACT. Surveillance of chronic wasting disease (CWD) was conducted by performing Western blot analysis of tissue samples from 136 sika deer (Cervus nippon) killed by hunters in the Tokachi district of Hokkaido Island. No prion protein (PrPSc) associated with CWD was detected in any of the samples. To assess amino acid polymorphisms of the sika deer PrP gene, nucleotide sequencing of the PrP gene was performed. The only amino acid polymorphisms detected were 3 silent mutations at nucleotide positions 63, 225 and 408. These results suggest that sika deer in the Tokachi district are genetically homogeneous, and are not infected with CWD.

KEY WORDS: CWD, sika deer, surveillance


FULL TEXT ;

http://www.jstage.jst.go.jp/article/jvms/67/3/349/_pdf

TSS




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