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From: TSS ()
Subject: Strain Typing of German Transmissible Spongiform Encephalopathies Field Cases in Small Ruminants by Biochemical Methods
Date: March 17, 2005 at 12:38 pm PST

-------- Original Message --------
Subject: [liste esb] Strain Typing of German Transmissible Spongiform Encephalopathies Field Cases in Small Ruminants by Biochemical Methods
Date: Thu, 17 Mar 2005 14:43:19 -0600
From: "Terry S. Singeltary Sr."
To: Bovine Spongiform Encephalopathy
CC: esb


Journal of Veterinary Medicine Series B
Volume 52 Issue 2 Page 55 - March 2005
doi:10.1111/j.1439-0450.2005.00827.x


Strain Typing of German Transmissible Spongiform Encephalopathies Field
Cases in Small Ruminants by Biochemical Methods
A. Gretzschel1
,
A. Buschmann1
,
M. Eiden1
,
U. Ziegler1
,
G. Lühken2
,
G. Erhardt2

and M. H. Groschup1,3


Summary

Following the implementation of a large scale transmissible spongiform
encephalopathies (TSE) surveillance programme of small ruminants,
evidence for a natural transmission of bovine spongiform encephalopathy
(BSE) to a French goat has been found. During the years 2002- 2004, a
massive TSE rapid testing programme on >250 000 small ruminants was
carried out in Germany. In this national survey, 186 scrapie-affected
sheep were found which originated from 78 flocks. The majority of these
cases were of the classical TSE type (115 sheep belonging to 14
outbreaks). However, 71 cases coming from 64 flocks were of the novel
atypical scrapie type. According to the regulation EU 999/2001, all TSE
cases in small ruminants have to be examined by strain typing methods to
explore any possibility of the existence of BSE cases in the field sheep
population. Here we report on a biochemical typing strategy (termed
FLI-test), which includes the determination of molecular masses,
antibody binding affinities and glycosylation pattern of the TSE induced
abnormal prion protein. Based on this typing approach none of the
analysed German classical TSE outbreaks (total number of analysed sheep:
36) displayed biochemical features indicative for a BSE infection.
However, in two cases distinct but BSE-unrelated PrPSc types were found,
which alludes to the existence of different scrapie strains in the
German sheep population.


Received for publication January 19, 2005


Affiliations

Addresses of authors: 1Friedrich-Loeffler-Institut (FLI), Institute for
Novel and Emerging Diseases, Insel Riems, Germany; 2Department of Animal
Breeding and Genetics, Justus-Liebig-University, Gießen, Germany;
3Corresponding author: E-mail: martin.groschup@rie.bfav.de

Correspondence

Friedrich-Loeffler-Institut, Institute for Novel and Infectious Emerging
Diseases, Boddenblick, Greifswald- Insel Riems, Germany
To cite this article
Gretzschel, A., Buschmann, A., Eiden, M., Ziegler, U., Lühken, G.,
Erhardt, G. & Groschup, M. H. (2005)
Strain Typing of German Transmissible Spongiform Encephalopathies Field
Cases in Small Ruminants by Biochemical Methods.
Journal of Veterinary Medicine Series B 52 (2), 55-63.
doi: 10.1111/
j.1439-0450.2005.00827.x


http://www.blackwell-synergy.com/links/doi/10.1111/j.1439-0450.2005.00827.x/abs/

TSS






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