From: TSS ()
Subject: Re: CONSUMER UNION ASKS FEDS TO RETEST SUSPECT MAD COW AFTER CRUCIAL TEST OMITTED !!!
Date: February 25, 2005 at 9:03 am PST
In Reply to: Re: CONSUMER UNION ASKS FEDS TO RETEST SUSPECT MAD COW AFTER CRUCIAL TEST OMITTED !!! posted by TSS on February 24, 2005 at 2:11 pm:
-------- Original Message --------
Subject: Re: Consumers Union asks feds to retest suspect mad cow after crucial test omitted
Date: Fri, 25 Feb 2005 10:34:44 -0600
From: "Terry S. Singeltary Sr."
To: Bovine Spongiform Encephalopathy
References: <421D4324.email@example.com> <421E4F9E.firstname.lastname@example.org>
Greetings list members,
I am wondering IF USDA/APHIS does go back and retest the mad cows
in TEXAS where they refused to use WB (they have those brains frozen),
I am curious since brains were frozen, will there be any problems from this
in there retesting? could this be used as an excuse by usda/aphis et al to
not test? also, IF they go back and retest those postive, positive,
inconclusive, negatives with same rapid tests at first as they did
before doing the WB as they should have in the first place, AND the
2 rapid test NOW come up negative after initial positives, would this
tell us that they are not the same brains or not? my devious mind is ticking
away here...you know curiosity killed the cat...
reason i am asking ;
> because brain tissue had not been stored in preservative and only
> frozen brain material was available which could not be used,
> SEAC 86/4
> Martin Jeffrey at VLA-Lasswade, Edinburgh has developed a
> immunohistochemistry (IHC) method to discriminate between BSE and
> scrapie in sheep. This method was recently shown to be discriminatory
> in an
> EU evaluation of discriminatory methods, together with two Western
> immunoblotting methods and an ELISA based-method. All of these methods
> are now recommended for use in the examination of samples that test
> in the EU-wide surveillance programme for TSEs in small ruminants. The
> Community Reference Laboratory’s Expert Group have agreed that none of
> the three in isolation is sufficient to confirm the presence of BSE
> and all three
> need to agree before a sample is confirmed as being indicative of BSE.
> has now been set out in a Commission Regulation1.
> Although IHC was not used to evaluate the recent French case of BSE in a
> goat, because brain tissue had not been stored in preservative and only
> frozen brain material was available which could not be used, the VLA have
> considered whether the method could be applied to goats as well as sheep.
> To achieve this the VLA examined brain tissue from goats that had been
> experimentally infected with BSE or scrapie, and compared them with two
> archived brain samples from scrapie-affected goats, namely:-
> • Five experimental BSE in goat samples provided by the IAH
> • Five SSBP-12 goat samples provided by the IAH (an experimentally
> transmitted isolate of scrapie)
> • Two historical samples of goat TSE (presumed scrapie) retained at VLA
> One of these latter samples, first reported to a veterinary centre in
> Scotland in
> 1990, has given an IHC result which is indistinguishable from the
> experimental BSE in goat samples (note – only the one method has been
> Because of the age of the sample the VLA only have fixed material. This
> means that they will not be able to perform any of the other molecular
> such as Western blot and ELISA. The only way that this sample can be
> confirmed to be from a BSE infection is for some of the fixed material
> to be
> inoculated into mice.
> VLA-Weybridge is also retrieving as many goat samples as it can
> identify in its
> archive to process in a similar fashion. Only eight goat TSE cases
> have been
> confirmed since January 1993 when scrapie became notifiable. None of the
> 1 Exact details of the Regulation can be found at
> 2 Sheep Scrapie Brain Pool 1 (SSBP-1) is a highly characterised pool
> of scrapie strains used
> by the IAH, which they routinely use in their experimental challenge work.
> SEAC 86/4
> examinations done so far have been sufficiently well peer-reviewed to
> the results to be reported, but none so far appear to look like BSE.
> Fallen Stock
> SEAC 86/5
> IMPLICATIONS OF THE FINDINGS OF HEIKENWALDER ET AL.
> 1. FSA has asked SEAC to consider the implications of the findings
> of a study by Heikenwalder et al. (2005)1.
> 2. Heikenwalder et al. (2005) report that in mouse models of
> inflammatory disease, the tissue distribution of prions is altered
> by chronic inflammation (Annex 1). In the study, five different
> mouse models were generated by genetic modification to induce
> chronic inflammation of the liver, kidney or pancreas (chronic
> inflammation of both the liver and pancreas was induced in two
> models). All the models expressed the murine prion protein
> gene. Following parenteral challenge with the scrapie agent
> (source unspecified) to each type of model, infectivity and
> abnormal prions were detected in the liver, kidney and/or
> pancreas in animals with chronic inflammation of these organs.
> In contrast, no or borderline infectivity and no abnormal prions
> were detected in these organs when wild-type mice were
> challenged with the scrapie agent.
> 3. The authors suggest that tissue inflammation may modify the
> tissue distribution of the infectious agent in prion diseases.
> ADVICE SOUGHT FROM THE COMMITTEE
> 4. The committee is asked to comment on the implications of the
> findings on the risk from the consumption of beef, or sheep and
> goat meat, in the light of current specified risk material controls.
> 1 Heikenwalder M, Zeller N, Seeger H, Prinz M, Klohn PC, Schwarz P,
> Ruddle NH,
> Weissmann C, Aguzzi A. Chronic Lymphocytic Inflammation Specifies the
> Organ Tropism of
> Prions. Science. 2005 Jan 20; [Epub ahead of print]
> SEAC 86/5
> ANNEX 1
> Heikenwalder et al. (2005) Chronic Lymphocytic Inflammation
> Specifies the Organ Tropism of Prions. Science.
Terry S. Singeltary Sr. wrote:
> ##################### Bovine Spongiform Encephalopathy
> GW SAYS DONT MESS WITH TEXAS OR ITS MAD COWS.
> SOME HISTORY ON THOSE TEXAS MAD COWS.
> United Press International: USDA vet: Texas mad cow breach not unique...
> Published 5/4/2004 5:01 PM WASHINGTON
> United Press International: USDA orders silence on mad cow in ...
> Published 5/11/2004 10:16 PM WASHINGTON
> Feds reviewing Texas mad cow breach - (United Press International)
> By Steve Mitchell United Press International.
> Washington, DC, May. 5 (UPI) -- The US Department ...
> No mad cow tests at Texas firm in 2004
> By Steve Mitchell
> United Press International
> Published 5/14/2004 11:06 AM
> WASHINGTON, May 14 (UPI) -- The U.S. Department of Agriculture did not
> test any cows for mad cow disease in the past seven months at the same
> Texas facility where federal testing policies for the deadly disorder
> were violated last month, United Press International has learned.
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