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From: TSS ()
Subject: Vermont Department of Health Statement to TSS about Belgium BSE sheep and CJD surveillance in Vermont
Date: February 23, 2005 at 2:01 pm PST

-------- Original Message --------
Subject: Re: AW: [BSE-L] USDA did not test possible mad cows - Dr. Detwiler, what about those sheep?
Date: Wed, 23 Feb 2005 14:12:27 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@LISTSERV.KALIV.UNI-KARLSRUHE.DE
References: <13.2d20eaae.2df84fb9@aol.com> <40C8C7A0.1080107@wt.net> <40CC806C.7010204@wt.net> <41C8727D.7090100@wt.net> <42015931.4010605@wt.net>


##################### Bovine Spongiform Encephalopathy #####################

Vermont Department of Health Statement to TSS about Belgium BSE sheep and CJD surveillance in Vermont

Vermont Department of Health

Division of Health Surveillance
Infectious Disease Epidemiology

Agency of Human Services

February 18,2005

Terry S. Singeltary Sr.

PO Box 42

Bacliff, Texas 77518

Dear Mr. Singeltary:

Thank you for your inquiry regarding the European sheep formerly in Vermont.
For an official USDA position regarding these sheep, please contact Ed Curlett at
301-734-3256.

As stated in your email, Vermont participates in a CDC -sponsored CJD
surveillance program. Although the CDC is particularly interested in deaths
among those younger than 55, the Vermont Department of Health looks for CJD-
related deaths among persons of any age. No CJD-related deaths have been
identified in the past two years.

Additionally, as part of our efforts to enhance surveillance for CJD, we recently
sent a letter to Vermont neurologists, requesting they send specimens to the
National Prion Disease Pathology Surveillance Center for testing as appropriate.

Sincerely,

Cort Lohff, MD, MPH
State Epidemiologist


108 Cherry Street • PO Box 70 • Burlington, VT 05402-0070 TEL 802-863-7240 or 800-640-4374
FAX 802-865-7701
====================

Greetings,


>Vermont participates in a CDC -sponsored CJD
>surveillance program

IT is of my opinion that the cdc et al do not want to know
the true depth of CJD/TSE in humans in the USA. to put
any age factor on surveillance with human TSEs is no scientific
approach to know the true prevelence of TSE
in humans. nvCJD has been documented in the elderly
(74 years oldest documented to date) and sporadic CJD has
been documented in teenagers. it's about like USDA et al
saying that there is no risk factor for BSE in cattle under
30 months of age. simply is not true. HUMAN TSEs must be made
reportable Nationally and Internationally.


>For an official USDA position regarding these sheep, please contact Ed Curlett at
>301-734-3256.


I will not be wasting my time with this. will not waste
the long distance money for the phone call for more lies.
why waste my time with more lies from ED ;


> Release No. 0141.02 Ed Curlett (301) 734-3256 Jerry
> Redding (202) 720-6959 TESTING TO CONTINUE ON IMPORTED SHEEP
> CONFISCATED LAST YEAR WASHINGTON, April 11, 2002

> The sheep were confiscated by USDA in March 2001 and
> transported to USDA's National Veterinary Services
> Laboratories in Ames, Iowa, where they were humanely euthanized.
> Tissue samples were collected from the sheep for diagnostic
> testing and USDA will continue with additional tests which
> could take up to 2 - 3 years to complete.

THE fact is, they lied about those mouse bio assays in
those Vermont sheep that most likely have BSE. NOW, the
question is, just how many of Vermonters consumed sheep
meat, milk and or cheese from those sheep that had BSE,
and if they will go clinical in there lifetime? WITH a
surveillance system only set up for humans younger than
55, the chance of finding any such cases is greatly diminished,
which is why the system was set up like that in the first place...


TSS SUBMISSION TO VERMONT HEALTH DEPT. ;

Health Alert on Certain Sheep Milk Cheese 2000 TSS SUBMISSION VERMONT HEALTH 2005

------------------------------------------------------------------------
-------- Original Message --------
Subject: CJD HUMAN/ANIMAL TSEs VERMONT i.e. ATYPICAL VERMONT SHEEP FROM
BELGIUM AND THAT CHEESE/MILK
Date: Sat, 05 Feb 2005 21:22:59 -0600
From: "Terry S. Singeltary Sr.
To: bapao@vdh.state.vt.us
CC: lcrist@vdh.state.vt.us ,
smoffat@vdh.state.vt.us

Greetings to

Vermont Department of Health

Division of Health Surveillance P.O. Box 70 Burlington, VT 05402-0070

Agency of Human Services

Jan K. Carney, MD, MPH

Commissioner

Ann R. Fingar, MD, MPH

State Epidemiologist Managing Editor

Apao,William K

Moffatt, Sharon G

I would like to write to you about my concerns of the most
recent findings of BSE to the GOAT under natural field
conditions and the recent findings of the Atypical Case of Bovine
Spongiform Encephalopathy in an East-Flemish Cow in Belgium,
in relations to the ATYPICAL VERMONT SHEEP and the
cheese and milk that was distributed to the public. THIS should
highten the concern of that situation, that was in all essence,
given the ALL CLEAR. I would also like to know why the
USDA put off those mouse bio assays of those sheep in
Vermont for 2 years, when we were told they had started?

July 19, 2000

Health Alert on Certain Sheep Milk Cheese

Based on advice from the Centers for Disease Control & Prevention, the
Vermont Health Department recommends that people not eat two brands of
Vermont sheep milk cheese.

(November 9, 2001: This Health Alert has been lifted.)

Retrieved on 2/5/05, NO link to original warning...TSS

http://www.healthyvermonters.info/news.shtml

Vermont Department of Health
Health Surveillance Division Reportable Diseases Updated 03/15/2004

Creutzfeldt-Jakob disease/transmissible
spongiform encephalopathies

snip...

Reporting of Diseases
The law requires that health care providers report diseases of public
health importance. Persons who are required to report: health care
facilities,
health care providers, health maintenance organizations, hospital
administrators, laboratory directors, managed care organizations, nurse
practitioners, nurses, physician assistants, physicians, school health
officials, town health officers. Cases of reportable diseases should be
reported to the Division within 24 hours.
24 Hour Telephone Reporting Line (802)951-4080 or 1-888-588-7781
Consultation and Inquiries 802-863-7240 (7:45AM  4:30PM M-F) or
1-800-640-4374 (VT only)
Emergency Consultation after normal business hours also available at
numbers above

http://www.healthyvermonters.info/hs/epi/idepi/reportable/CommunicableDiseaseRegs2004.pdf


1. Reportable Diseases and Syndromes (to include any rare infectious
disease or one dangerous to public health)
Any unexpected pattern of cases, suspected cases, deaths or increased
incidence of any other illness of major
public health concern, because of the severity of illness or potential
for epidemic spread, which may indicate a newly
recognized infectious agent, an outbreak, epidemic, related public
health hazard or act of bioterrorism.

snip...

Creutzfeldt-Jakob disease/transmissible
spongiform encephalopathies

snip...

http://www.healthyvermonters.info/hs/epi/idepi/reportable/CommunicableDiseaseRegs2004.pdf

FULL TEXT FOLLOW THREAD

http://disc.server.com/discussion.cgi?disc=167318;article=1926;title=CJD%20WATCH

TSS

http://www.prwatch.org/forum/showthread.php?p=12566#post12566

BASEd on all this and THE FINDINGS OF ATYIPCAL TSE IN BELGIUM COW,
makes you wonder just what those Vermont Belgium sheep really had just
what tissues
are infectious to humans;

Atypical Case of Bovine Spongiform Encephalopathy in an East-Flemish
Cow in Belgium

H. De Bosschere, DVM, PhD

S. Roels, DVM, PhD

E. Vanopdenbosch, DVM, Lic

Veterinary and Agrochemical Research Centre (CODA/CERVA)

National Reference Laboratorium for Veterinary TSEs

Groeselenberg 99, B-1180

Ukkel (Brussels), Belgium

KEY WORDS: Bovine spongiform encephalopathy, BSE, Western blot, atypical
BSE.

ABSTRACT

For many years, researchers believed that only one bovine spongiform
encephalopathy (BSE) strain existed, in contrast to the many different
scrapie strains found. However, only very recently reports emerged about
unconventional BSE strains seen in Italy, France, and Japan. The present
case describes an atypical strain of BSE in Belgium in a 64-month-old
East-Flemish cow with an electrophoretic profile and other features
similar to those described in Japan.

INTRODUCTION

snip...

DISCUSSION

For many years, researchers assumed that only one BSE strain
existed.710 Only in the past months, reports of atypical BSE cases
were announced.1113 The Japanese case11 describes a very young bull (23
months) characterized by the absence of spongiform changes and PrPsc
deposits immunohistochemically. The WB analysis revealed an
electrophoretic profile different from that of typical BSE,
characterized by low content of the di-glycosylated molecular form of
PrPsc and a faster migration of the nonglycosylated form of PrPsc. In
Italy,12 two BSE affected cattle with a previously unrecognized
neuropathologic profile and PrPsc type were seen. These cases were
determined using a different staining pattern on immunohistochemistry, a
difference in size and glycoform ratio of PrPsc on immunoblot and a
difference in regional distribution of lesions. The two cases in
France13 showed variant molecular features with a different PrPsc
electrophoretic profile from other BSE cases, mainly characterized by a
higher molecular mass of the nonglycosylated PrPsc. The present case
shows the most similarities (ie, identical electrophoretic profile, only
ELISA and WB positive and histopathology and immunohistochemistry
negative) with the Japanese case,11 although the cow in the Japanese
case was only 23 months old, and the cow in this case was 64 months old.

The fact that these strains were detected worldwide and in several
breeds suggest that there is no local or breed-dependent feature
involved. It could be that the WB techniques have become more specific
within the past year in the detection of minor differences in di-,
mono-, and nonglycosylated molecular forms of PrPsc. Infection of cattle
by scrapie could also be considered since scrapie can be transmitted by
direct contact between animals or through environmental contamination.13

In conclusion, this Belgian case should be added to the list of atypical
BSE strains only very recently detected worldwide and may contribute to
further research studies about epidemiologic significance. Current
continued research on BSE would appear to reveal different BSE strains
in analogy with the different scrapie strains.

ACKNOWLEDGMENTS

snip...


http://www.jarvm.com/articles/Vol2Iss1/DEBOSSCHERE.htm

NOT TO FORGET THIS FOR SURE;

BASE in cattle in Italy of Identification of a second bovine amyloidotic
spongiform
encephalopathy: Molecular similarities with sporadic Creutzfeldt-Jakob
disease

http://www.pnas.org/cgi/content/abstract/0305777101v1

Adaptation of the bovine spongiform encephalopathy agent to primates
and comparison with Creutzfeldt- Jakob disease:
Implications for human health

THE findings from Corinne Ida Lasmézas*, [dagger] , Jean-Guy Fournier*,
Virginie Nouvel*, Hermann Boe*, Domíníque Marcé*, François Lamoury*,
Nicolas Kopp [Dagger ] , Jean-Jacques Hauw§, James Ironside¶, Moira
Bruce [||] ,
Dominique Dormont*, and Jean-Philippe Deslys* et al, that The agent
responsible for
French iatrogenic growth hormone-linked CJD taken as a control is very
different
from vCJD but is similar to that found in one case of sporadic CJD and
one sheep scrapie isolate;

http://www.pnas.org/cgi/content/full/041490898v1

Characterization of two distinct prion strains derived from bovine
spongiform encephalopathy
transmissions to inbred mice

http://vir.sgmjournals.org/cgi/content/abstract/85/8/2471


THE findings from the cow in Japan with tissue infectivity in the
peripheral nerve tissue, suprarenal gland,
First time from non-Specified Risk Material ;


Japan Consumer Press online
Nippon shouhisha shinbun
http://www.jc-press.com/eg.htm
http://www.jc-press.com/En/Latest%20News/200411/20041109BSE%20death%20cow%27s%20anomalous%20prion.htm
Last modified, 11/09/2004 13:42:49
BSE death cow's anomalous prion detected from peripheral nerve tissue,
suprarenal gland
First time from non-Specified Risk Material, or SRM
By JCPRESS
National Institute of Animal Health Animal announced on November 1 that
it had detected the anomalous prion protein that was the etiologic agent
of the mad cow disease, or BSE, or bovine spongiform encephaalopathy,
from the peripheral nerve tissue and the suprarenal gland of the cow of
the age in the mad cow disease for the dying infection 94 months on
March 9 this year.
Japan is obligating the removal of the Specified Risk Material, or SRM
such as the head, the spinal cord, the vertebral columns, and the small
intestines that accumulate the anomalous prion protein easily as a BSE
(bovine spongiform encephaalopathy) measures.
Because the mad cow disease etiologic agent was detected from a tissue
different from the Specified Risk Material, or SRM, the review of the
Specified Risk Material, or SRM might be urged on the Japanese Government.
International Symposium of PRION DISEASES for food and drug safety
http://www.knt.co.jp/ec/2004/prion/
national institute of animal health(only in Japanese)
http://niah.naro.affrc.go.jp/index-j.html
The statement of the Ministry of Health, Labour and Welfare
(only in Japanese)
http://www.maff.go.jp/www/press/cont2/20041101press_7.htm
Yomiuri on line (only in Japanese)
http://www.yomiuri.co.jp/science/news/20041102i503.htm
Asahi on line(only in Japanese)
http://www.asahi.com/special/bse/TKY200411010291.html
Mainichi on line(only in Japanese)
http://www.mainichi-msn.co.jp/shakai/jiken/disease/news/20041102ddm041040128000c.html


WITH all this TSE SCIENCE, i would just sit around and flounder another
2-3 years
on those mouse bio assays on those Vermont sheep, deny that any of it
ever happened,
and by all means implement GWs Minimal Risk Region for TSEs (MRR) or
what i call
the Legal Trading of TSEs (MRRLTTSE) POLICY for short, flounder a little
longer,
continue to use the SSS policy of Canada's on any other mad cows in
Texas or any where
else, then just pass the buck off to the next administration. problem
solved. been going on
for decades...


still disgusted in Bacliff, Texas USA...TSS


Terry S. Singeltary Sr. wrote:

> ##################### Bovine Spongiform Encephalopathy
> #####################
>
> Greetings list members,
>
> THIS confirmed BSE in GOAT by NATURAL conditions ;
>
> A suspected case of BSE in a goat slaughtered in France in 2002 has
> been confirmed today by a panel of European scientists
>
> http://europa.eu.int/rapid/pressReleasesAction.do?reference=MEMO/05/29&format=HTML&aged=0&language=EN&guiLanguage=en
>
>
> http://europa.eu.int/comm/food/food/biosafety/bse/crl_statement_tse_goats_28-01-05_en.pdf
>
>
> SURE makes these ATYPICAL TSE sheep from VERMONT a bit
> more interesting. MAYBE this is why the mouse bio assays were
> put off for more than 2 years, and nobody knows why or anything
> about it ;-)
>
> TSS
>
> Terry S. Singeltary Sr. wrote:
>
>> ##################### Bovine Spongiform Encephalopathy
>> #####################
>>
>> Greetings list members,
>>
>> It seems my efforts to find the truth behind the lies the USDA keep
>> telling us about the animal TSEs in the USA just keep getting deeper
>> and deeper. IT's like a revolving door of lies and deceit about
>> mad cow disease and other TSEs in animals in the USA. I would say
>> watching this administration crumble before our eyes after the
>> re-election, i would say these people deserve every bit that they get,
>> they voted for this chronic liar, put him back in office for us all to
>> endure another 4 years of the same old BSeee. HOWEVER, after
>> great consideration and thought i am deeply saddened at all this.
>> WHERE does it all end? HOW far is this administration willing
>> to go? IT's really become very very frightening. I feel like we are
>> not living in a Democracy anymore, but under a Dictatorship.
>> Everyone is entitled to an opinion, and that is mine.
>>
>> WITH that said, I got a reply finally from SEAC about the infamous
>> VERMONT sheep from Belgium with the atypical TSE.
>>
>> IF you remember correctly, Dr. Detwiler kindly replied to me about
>> this ;
>>
>>> 6/12/04
>>>
>>> Mr. Singeltary.
>>
>>
>>
>>> I hope this finds you well. As you are aware I left the USDA last
>>> year. I can only update you on the sheep before that time. Contact
>>> was established with the UK on doing the bioassay studies. They
>>> agreed. However, we were prioritized after their own needs, hence
>>> the delay. I am aware that there are now additional labs in Europe
>>> running the mouse bioassay strain typing. You will have to contact
>>> USDA for further word.
>>>
>>>>
>>>>
>>>> Linda Detwiler
>>>
>>>
>>>
>>
>> TODAY, i finally recieve this from SEAC about these so called
>> mouse bioassays that never took place on the Vermont sheep,
>> and it still looks like the ball was dropped by the USDA to me.
>> I guess like everything else they do, we will never know the truth...
>>
>>
>> -------- Original Message --------
>> Subject: re-85th Meeting of SEAC - 30.11.04
>> Date: Tue, 21 Dec 2004 16:56:55 -0000
>> From: "Barlow, Tom (SEAC)"
>> To: "'flounder@wt.net'"
>>
>>
>>
>> Dear Mr Singeltary
>>
>> Thank you for you enquiry to the SEAC secretariat about mouse bioassays
>> commissioned by the USDA to investigate TSE cases in imported sheep.
>

>> After making a number of enquiries, it appears that Defra were not
>> involved
>> with this work. However, it is possible that a UK research
>> laboratory was
>> contacted by the USDA about such tests but I have been unable to find
>> out
>> any further information. You may wish to make further enquiries with
>> the
>> USDA.
>>
>> Yours sincerely
>>
>> Tom Barlow
>>
>> Dr Tom Barlow
>> Spongiform Encephalopathy Advisory Committee (SEAC) Secretariat
>> Area 108, 1A Page Street, London SW1P 4PQ
>>
>> Tel: 0207 904 6267
>> -----Original Message-----
>> From: Terry S. Singeltary Sr. [mailto:flounder@wt.net]
>> Sent: 02 December 2004 20:19
>> To: Dale, Tabitha J (SEAC)
>> Subject: re-85th Meeting of SEAC - 30.11.04
>>
>>
>> Hello Tabitha,
>>
>> A kind greetings from Texas.
>>
>> I had signed up for the meeting and wanted to ask a
>> question, but it took
>> me too long to finally get everything working properly on my
>> end with
>> the viewing. finally got things going today and got into the
>> audio of the
>> meeting (will have to download an upgrade for my windows
>> media).
>>
>> ASIDE from the disturbing points made about sCJD not being
>> tied
>> to BSE from some unpublished mouse bioassays (if i heard
>> that right)
>> and the fact that they still today base the increase of
>> sporadic CJD in
>> known BSE countries as a happenstance of better
>> surveillance, I wish
>> to kindly ask a question not pertaining to the above, as
>> disturbing as
>> it is (lost my mother to the hvCJD 12/14/97) and i simply
>> have never
>> accepted the spontaneous/sporadic aspects of this agent in
>> 85%+ of
>> all humans. never will, it's a pipe dream thought up in some
>> back room
>> in the 80s to protect the industries involved (my opinion).
>>
>> MY question is one about the VERMONT USA SHEEP that were
>> imported to the USA from Belgium and confiscated by the USDA
>> several
>> years ago due to an atypical TSE, with the announcement that
>> mouse studies would be immediately started. I was informed by
>> Dr.
>> Linda Detwiler that it
>> was DEFRA that was responsible for these mouse studies being
>> put on hold
>> for 2 years. WHY were such important studies put off for 2
>> years by DEFRA?
>>
>> HERE is my correspondence with Dr. Detwiler;
>>
>> Release No. 0141.02
>>
>> Ed Curlett (301) 734-3256
>> Jerry Redding (202) 720-6959
>>
>>
>> TESTING TO CONTINUE ON IMPORTED SHEEP CONFISCATED LAST YEAR
>>
>>
>> WASHINGTON, April 11, 2002 -- The U.S. Department of
>> Agriculture today announced that tests conducted on a flock
>> of sheep
>> confiscated last year from a farm in Vermont confirm that two
>> of the 125 sheep
>> tested positive for an atypical undifferentiated
>> transmissible spongiform
>> encephalopathy (TSE) of foreign origin. The flock of 125
>> sheep was
>> confiscated in March 2001 after four animals from an
>> associated flock tested
>> positive for TSE in July 2000. USDA will continue to conduct
>> additional tests
>> to determine the type of TSE in these sheep.
>>
>> "These results confirm our previous conclusions were correct
>> and that we took the appropriate preventative actions in
>> confiscating
>> these animals," said Bobby Acord, administrator of USDAs Animal
>> and Plant Health Inspection Service. "USDAs actions to
>> confiscate,
>> sample and destroy these sheep were on target. As a result of
>> our
>> vigilance, none of these confiscated animals entered the
>> animal or human
>> food supply."
>>
>> The sheep, imported from Belgium and the Netherlands in
>> 1996, were placed under certain federal restrictions when
>> they entered
>> the country as part of USDA's scrapie control efforts. In
>> 1998, USDA
>> learned that it was likely that sheep from Europe were
>> exposed to feed
>> contaminated with bovine spongiform encephalopathy. At that
>> time, the state of
>> Vermont, at the request of USDA, imposed a quarantine on
>> these flocks,
>> which prohibited slaughter or sale for breeding purposes.
>>
>> On July 10, 2000, several sheep from the flock tested
>> positive for a TSE, a class of degenerative neurological
>> diseases that is
>> characterized by a very long incubation period and a 100
>> percent mortality
>> rate in infected sheep. Two of the better known varieties of
>> TSE are
>> scrapie in sheep and BSE in cattle. There is no evidence that
>> scrapie
>> poses a risk to human health.
>>
>> On July 14, 2000, USDA issued a declaration of extraordinary
>> emergency to acquire the sheep. This action was contested by
>> the flock
>> owners. A federal district court judge ruled in favor of USDA
>> based on
>> the merits of the case. The flock owners appealed to the
>> Second Circuit
>> Court requesting a stay, which was denied. The sheep were
>> confiscated by USDA in March 2001 and transported to USDA's
>> National Veterinary
>> Services Laboratories in Ames, Iowa, where they were humanely
>> euthanized. Tissue samples were collected from the sheep for
>> diagnostic testing
>> and USDA will continue with additional tests which could take
>> up to 2
>> - 3 years to complete. In all, USDA has acquired 380 sheep
>> from a
>> total of three flocks. All of the animals were humanely
>> euthanized, sampled
>> and disposed and did not enter the animal or human food supply.
>>
>> Our goal continues to be to prevent, detect and eradicate
>> foreign animal diseases to protect American agriculture,
>> natural resources
>> and consumers," said Acord. "We will continue to utilize the
>> scientific results of these and other tests conducted during
>> the last
>> several years to strengthen our extensive surveillance,
>> monitoring and
>> prevention efforts."
>>
>> For more information about USDAs ongoing surveillance,
>> monitoring and prevention efforts as it relates to this
>> situation, please
>> visit www.aphis.usda.gov/oa/tse/index.html
>>
>>
>> #
>>
>>
>> NOW, June 2004 those same test that we were told would start
>> in
>> 2002, have yet to be started. THE TSE those VERMONT sheep
>> was supposedly to have had, has yet to be confirmed.
>>
>> WHY?
>>
>> Correspondence from Dr. Detwiler to me;
>>
>> -------- Original Message --------
>> Subject: Sheep
>> Date: Sat, 12 Jun 2004 14:26:04 EDT
>> From: LAVET22@aol.com
>> To: flounder@wt.net
>>
>>
>>
>> Mr. Singeltary.
>>
>> I hope this finds you well. As you are aware I left the
>> USDA last
>> year. I can only update you on the sheep before that time.
>> Contact was
>> established with the UK on doing the bioassay studies. They
>> agreed.
>> However, we were prioritized after their own needs, hence
>> the delay. I
>> am aware that there are now additional labs in Europe
>> running the mouse
>> bioassay strain typing. You will have to contact USDA for
>> further word.
>>
>>
>> Linda Detwiler
>> =========
>>
>>
>> >However, we were prioritized after their own needs, hence
>> the delay. >
>>
>> IF this was the case, this is totally unacceptable. FOR
>> something that has been
>> ongoing since the 80s (BSE in SHEEP/GOATS) yet still
>> unresolved, there is
>> absolutely no excuse why these studies were put off. with
>> the other sheep
>> brain mix-up and now the BSE in the French Goat, I find it
>> very disturbing that
>> the Vermont Sheep studies were put off for 2 years for
>> whatever reason, especially with the findings Dormont*, and
>> Jean-Philippe
>> Deslys* et al, that
>> The agent responsible for French iatrogenic growth
>> hormone-linked CJD taken as a control is very different from
>> vCJD but is
>> similar to that found in one case of sporadic CJD and one
>> sheep scrapie
>> isolate;
>>
>> http://www.pnas.org/cgi/content/full/041490898v1
>>
>>
>> YES, i am still very angry, but i want to still thank SEAC
>> for the work
>> they have done, i only wish things would go much faster and
>> that the
>> BSE/nvCJD only theory would be put to rest once and for all.
>> Science
>> has pretty much proven that it was a pipe dream, however
>> science
>> does not have near as much to do with this mess anymore as
>> the
>> industry and politics do. it's simply not about science
>> anymore.
>> IN the USA, you dont even hear of these new studies from the
>> Gov. and very little from the media...
>>
>> thank you,
>> kindest regards,
>>
>> Terry S. Singeltary Sr.
>> P.O. Box 42
>> Bacliff, TEXAS USA 77518
>>
>>
>> The original of this email was scanned for viruses by the Government
>> Secure Intranet (GSi) virus scanning service supplied exclusively by
>> Energis in partnership with MessageLabs.
>>
>> On leaving the GSi this email was certified virus-free
>>
>>
>>
>>
>>
>> Terry S. Singeltary Sr. wrote:
>>
>>> ######## Bovine Spongiform Encephalopathy
>>> #########
>>>
>>> Greetings list members,
>>>
>>> Thought I should let the list know that Dr. Detwiler kindly replied
>>> to my
>>> question about the delayed 'atypical' TSE testing in the Vermont
>>> sheep and
>>> tried to explain what caused the delay. If I interpreted it correctly,
>>> seems it was the fault of the U.K. ;
>>>
>>> -------- Original Message --------
>>> Subject: Sheep
>>> Date: Sat, 12 Jun 2004 14:26:04 EDT
>>> From: LAVET22@aol.com
>>> To: flounder@wt.net
>>>
>>>
>>>
>>> Mr. Singeltary.
>>>
>>> I hope this finds you well. As you are aware I left the USDA last
>>> year. I can only update you on the sheep before that time. Contact
>>> was
>>> established with the UK on doing the bioassay studies. They agreed.
>>> However, we were prioritized after their own needs, hence the delay. I
>>> am aware that there are now additional labs in Europe running the mouse
>>> bioassay strain typing. You will have to contact USDA for further
>>> word.
>>>
>>>
>>> Linda Detwiler
>>> =========
>>>
>>> My reply to Dr. Detwiler;
>>>
>>>
>>> -------- Original Message --------
>>> Subject: Re: Sheep
>>> Date: Sat, 12 Jun 2004 13:53:57 -0500
>>> From: "Terry S. Singeltary Sr."
>>> To: LAVET22@aol.com
>>> References: <54.2bd2ac1e.2dfca4bc@aol.com>
>>>
>>>
>>>
>>> hello Dr. Detwiler,
>>>
>>> thanks for your kind reply.
>>>
>>>> However, we were prioritized after their own needs, hence the delay.
>>>
>>>
>>>
>>>
>>>
>>> not sure i understand that?
>>>
>>>> You will have to contact USDA for further word.
>>>
>>>
>>>
>>>
>>>
>>> already done that, and there answer was;
>>>
>>>> 5/20/04
>>>>
>>>
>>>> Dear Mr. Singeltary,
>>>>
>>>> The Western blot tests on these animals were completed in April of
>>>> this
>>>> year. That means that we can begin the mouse inoculations. To get the
>>>> results of the Western blot tests, you will need to submit a
>>>> Freedom of
>>>> Information Act request through our FOIA office. The FAX number
>>>> there is
>>>> 301-734-5941.
>>>>
>>>> Have a nice day,
>>>>
>>>> Jim Rogers
>>>> APHIS LPA
>>>>
>>>
>>> and with my previous attempts for information via the FOIA through
>>> this administration (as you are probably very well aware of) they have
>>> all been ignored/refused. so any further attempts would be fruitless
>>> i am
>>> sure.
>>>
>>> thanks anyway...
>>>
>>> kindest regards,
>>> Terry
>>>
>>> LAVET22@aol.com wrote:
>>>
>>>> Mr. Singeltary.
>>>
>>>
>>>
>>>
>>> snip...
>>>
>>> TSS
>>>
>>> Terry S. Singeltary Sr. wrote:
>>>
>>>> ######## Bovine Spongiform Encephalopathy
>>>> #########
>>>>
>>>> Greetings Dr. Detwiler,
>>>>
>>>> glad to see you are still with us, you had become very silent lately.
>>>> hope you are enjoying semi retirement.
>>>>
>>>> recently, i inquired through the BSE-L and via USDA official about
>>>> those Vermont sheep via belgium which there was an Extraordinary
>>>> Declaration of Emergency declared here in the USA due to
>>>> atypical scrapie. The thread is;
>>>>
>>>> Confiscation of Sheep in Vermont and testing results ? Thu, 20 May
>>>> 2004
>>>> 12:10:03 -0500 "Terry S. Singeltary Sr." Bovine
>>>> Spongiform Encephalopathy BSE-L
>>>>
>>>>
>>>>
>>>>> Imported
>>>>> Belgium/Netherlands
>>>>> Sheep Test Results
>>>>> Background
>>>>> Factsheet
>>>>> Veterinary Services April 2002
>>>>> APHIS
>>>>
>>>>
>>>>
>>>>
>>>>
>>>>
>>>> snip...
>>>>
>>>>> Additional tests will be conducted to determine
>>>>> exactly what TSE the animals haveBSE or scrapie.
>>>>> These tests involve the use of bioassays that consist
>>>>> of injecting mice with tissue from the infected animals
>>>>> and waiting for them to develop disease. This testing
>>>>> may take at least 2 to 3 years to complete.
>>>>
>>>>
>>>>
>>>>
>>>>
>>>>
>>>> http://www.aphis.usda.gov/lpa/pubs/fsheet_faq_notice/fs_ahvtsheeptr.pdf
>>>>
>>>>
>>>> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E.
>>>> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
>>>>
>>>> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci
>>>>
>>>>
>>>> d=fr20jy00-32
>>>>
>>>>
>>>>
>>>> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
>>>> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
>>>>
>>>> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci
>>>>
>>>>
>>>> d=fr20jy00-31
>>>>
>>>>
>>>>
>>>> or if those old urls dont work, go here;
>>>>
>>>> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
>>>> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
>>>> - Terry S.
>>>> Singeltary Sr. 7/20/00 (0)
>>>>
>>>> http://www.vegsource.com/talk/madcow/messages/7507.html
>>>>
>>>> I was told that ;
>>>>
>>>>
>>>> -------- Original Message --------
>>>> Subject: Re: hello Dr. Sutton...question please...scrapie...TSS
>>>> Date: Thu, 20 May 2004 14:36:09 -0400
>>>> From: Jim.D.Rogers@aphis.usda.gov
>>>> To: flounder@wt.net
>>>>
>>>>
>>>>
>>>> Dear Mr. Singeltary,
>>>>
>>>> The Western blot tests on these animals were completed in April of
>>>> this
>>>> year. That means that we can begin the mouse inoculations. To get the
>>>> results of the Western blot tests, you will need to submit a
>>>> Freedom of
>>>> Information Act request through our FOIA office. The FAX number
>>>> there is
>>>> 301-734-5941.
>>>>
>>>> Have a nice day,
>>>>
>>>> Jim Rogers
>>>> APHIS LPA
>>>> =========
>>>>
>>>>
>>>> Dr. Detwiler, my question is, why have these very important test been
>>>> delayed for so long when we were told they were to have been started
>>>> some 2+ years ago?
>>>>
>>>> who made this call to delay these very important test and why ?
>>>>
>>>> thank you,
>>>> with kindest regards,
>>>>
>>>> Terry
>>>>
>>>>
>>>> Linda Detwiler wrote:
>>>>
>>>>> ######## Bovine Spongiform Encephalopathy
>>>>> #########
>>>>>
>>>>> I m responding to Roland's post about my quote in the article by
>>>>> Steve
>>>>> Mitchell. I spent a fair amount of time on the phone with Mr.
>>>>> Mitchell on more than
>>>>> one occasion. The quote was one aspect of our conversation. Even
>>>>> the quote
>>>>> included "probably". I explained about proper location and sampling
>>>>> condition of the brain. I also added in our conversation that the
>>>>> best methodology is
>>>>> to utilize both a test for PrP as well as histopathology when
>>>>> examining
>>>>> brains from cattle with CNS disease. This is why as early as
>>>>> 1993-94 the USDA
>>>>> began using IHC in its TSE testing regime at the National Veterinary
>>>>> Services
>>>>> Laboratory. However, utilizing only a PrP test eliminates the
>>>>> possibility of
>>>>> diagnosing another neurologic disease.
>>>>>
>>>>> All of the tests have advantages and disadvantages. For example, I
>>>>> have been
>>>>> to a number of laboratories in Europe and watched as technicians took
>>>>> the
>>>>> test samples from the brain stem. They sample hundreds per night.
>>>>> If they get
>>>>> distracted the may take the sample lateral, or rostral to the
>>>>> obex. If this
>>>>> animal was in an earlier stage of disease, there may be a very small
>>>>> amount of
>>>>> PrPsc and limited to a single location in the brain. If that sample
>>>>> missed
>>>>> the obex, the sample would be negative and life goes on. With any
>>>>> test using
>>>>> a homogenate I am not aware that there is any cross check for
>>>>> location.
>>>>>
>>>>> With IHC, the pathologist can determine location, however it too has
>>>>> drawbacks in regard to sample condition. There is no perfect test.
>>>>> There are
>>>>> limitations to the tests themselves and there are limitations to all
>>>>> of the aspects
>>>>> of collection. Hence utilizing multiple tests especially for CNS
>>>>> cases is
>>>>> prudent. It is also prudent to examine other locations of the brain
>>>>> in the event
>>>>> a disease changes or something new emerges.
>>>>>
>>>>>
>>>>> Linda Detwiler
>>>>>
>>>>> ######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>>>>> ##########
>>>>>
>>>>>
>>>>>
>>>>
>>>> ######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>>>> ##########
>>>>
>>>
>>> ######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>>> ##########
>>>
>> TSS
>>
>> ############## BSE-L-subscribe-request@kaliv.uni-karlsruhe.de
>> ##############
>>
>>
>
> ######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html
> ##########
>
>

######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########






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