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From: TSS ()
Subject: Phenotype of disease-associated PrP accumulation in the brain of bovine spongiform encephalopathy experimentally infected sheep
Date: February 19, 2005 at 9:09 am PST

-------- Original Message --------
Subject: Phenotype of disease-associated PrP accumulation in the brain of bovine spongiform encephalopathy experimentally infected sheep
Date: Fri, 18 Feb 2005 20:12:47 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@LISTSERV.KALIV.UNI-KARLSRUHE.DE


##################### Bovine Spongiform Encephalopathy #####################

------------------------------------------------------------------------
J Gen Virol 86 (2005), 827-838; DOI 10.1099/vir.0.80299-0

Phenotype of disease-associated PrP accumulation in the brain of
bovine spongiform encephalopathy experimentally infected sheep


Lorenzo González1, Stuart Martin1, Fiona E. Houston2, Nora Hunter3, Hugh
W. Reid4, Sue J. Bellworthy5 and Martin Jeffrey1

1 Veterinary Laboratories Agency (VLA-Lasswade), Pentlands Science Park,
Bush Loan, Penicuik, Midlothian EH26 0PZ, UK
2 Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
3 Institute for Animal Health Neuropathogenesis Unit, Edinburgh EH9 3JF, UK
4 Moredun Research Institute, Pentlands Science Park, Bush Loan,
Penicuik, Midlothian EH26 0PZ, UK
5 VLA-Weybridge, Addlestone, Surrey KT15 3NB, UK

Correspondence
Lorenzo González
l.gonzalez@vla.defra.gsi.gov.uk

In view of the established link between bovine spongiform encephalopathy
(BSE) and variant Creutzfeldt–Jakob disease and of the susceptibility of
sheep to experimental BSE, the detection of potential cases of naturally
occurring BSE in sheep has become of great importance. In this study,
the immunohistochemical (IHC) phenotype of disease-associated prion
protein (PrPd) accumulation has been determined in the brain of 64
sheep, of various breeds and PrP genotypes, that had developed
neurological disease after experimental BSE challenge with different
inocula by a range of routes. Sheep BSE was characterized by
neuron-associated intra- and extracellular PrPd aggregates and by
conspicuous and consistent deposits in the cytoplasm of microglia-like
cells. The stellate PrPd type was also prominent in most brain areas and
marked linear deposits in the striatum and midbrain were distinctive.
Sheep of the ARR/ARR and ARQ/AHQ genotypes displayed lower levels of
PrPd than other sheep, and intracerebral BSE challenge resulted in
higher levels of PrPd accumulating in the brain compared with other
routes. The PrP genotype and the route of challenge also appeared to
affect the incubation period of the disease, giving rise to complex
combinations of magnitude of PrPd accumulation and incubation period.
Despite these differences, the phenotype of PrPd accumulation was found
to be very consistent across the different factors tested (notably after
subpassage of BSE in sheep), thus highlighting the importance of
detailed IHC examination of the brain of clinically affected sheep for
the identification of potential naturally occurring ovine BSE.


http://vir.sgmjournals.org/cgi/content/abstract/86/3/827?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=prion&searchid=1108778143595_1188&stored_search=&FIRSTINDEX=0&volume=86&issue=3&search_url=http%3A%2F%2Fvir.sgmjournals.org%2Fcgi%2Fsearch


TSS

######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########






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