SEARCH VEGSOURCE:

 

 

Follow Ups | Post Followup | Back to Discussion Board | VegSource
See spam or
inappropriate posts?
Please let us know.
  




From: TSS ()
Subject: An enzyme–detergent method for effective prion decontamination of surgical steel
Date: February 19, 2005 at 9:03 am PST

-------- Original Message --------
Subject: An enzyme–detergent method for effective prion decontamination of surgical steel
Date: Fri, 18 Feb 2005 20:14:06 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@LISTSERV.KALIV.UNI-KARLSRUHE.DE


##################### Bovine Spongiform Encephalopathy #####################

© 2005 Society for General
Microbiology


An enzyme–detergent method for effective prion decontamination of
surgical steel

Graham S. Jackson, Edward McKintosh, Eckhard Flechsig{dagger} , Kanella
Prodromidou, Petra Hirsch, Jackie Linehan, Sebastian Brandner, Anthony
R. Clarke, Charles Weissmann and John Collinge

MRC Prion Unit, Department of Neurodegenerative Disease, Institute of
Neurology, University College London, Queen Square, London WC1N 3BG, UK

Correspondence
John Collinge
j.collinge@prion.ucl.ac.uk

Prions, transmissible agents that cause Creutzfeldt–Jakob disease (CJD)
and other prion diseases, are known to resist conventional sterilization
procedures. Iatrogenic transmission of classical CJD via neurosurgical
instruments is well documented and the involvement of lymphoreticular
tissues in variant CJD (vCJD), together with the unknown population
prevalence of asymptomatic vCJD infection, has led to concerns about
transmission from a wide range of surgical procedures. To address this
problem, conditions were sought that destroy PrPSc from vCJD-infected
human tissue and eradicate RML prion infectivity adsorbed onto surgical
steel. Seven proteolytic enzymes were evaluated individually and in
pairs at a range of temperatures and pH values and the additional
effects of detergents, lipases and metal ions were assessed. A
combination of proteinase K and Pronase, in conjunction with SDS, was
shown to degrade PrPSc material from highly concentrated vCJD-infected
brain preparations to a level below detection. When RML prion-infected
wires were exposed to the same enzymic treatment, intracerebral bioassay
in highly susceptible hosts showed virtually no infectivity. The
prion-degrading reagents identified in this study are readily available,
inexpensive, non-corrosive to instruments, non-hazardous to staff and
compatible with current equipment and procedures used in hospital
sterilization units.

{dagger} Present address: Institute for Virology and Immunobiology,
D-97078 Würzburg, Germany.

http://vir.sgmjournals.org/cgi/content/abstract/86/3/869?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=prion&searchid=1108778143595_1188&stored_search=&FIRSTINDEX=0&volume=86&issue=3&search_url=http%3A%2F%2Fvir.sgmjournals.org%2Fcgi%2Fsearch

TSS

######### https://listserv.kaliv.uni-karlsruhe.de/warc/bse-l.html ##########





Follow Ups:



Post a Followup

Name:
E-mail: (optional)
Subject:

Comments:

Optional Link URL:
Link Title:
Optional Image URL: