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From: TSS (216-119-132-57.ipset12.wt.net)
Subject: Experimental Inoculation of Tme, Scrapie, and Cwd to Raccoons: An Update Authors
Date: November 7, 2004 at 6:46 pm PST

-------- Original Message --------
Subject: Experimental Inoculation of Tme, Scrapie, and Cwd to Raccoons: An Update Authors
Date: Sun, 07 Nov 2004 20:46:33 -0600
From: "Terry S. Singeltary Sr."
To: BSE-L , CJDVOICE@YAHOOGROUPS.COM

Research

Research >

Title: Experimental Inoculation of Tme, Scrapie, and Cwd to Raccoons: An
Update Authors
item Hamir, Amirali

item Miller, Janice - ARS RETIRED
item Cutlip, Randall - ARS RETIRED
item Stack, Mick - VLA-WEYBRIDGE, UK
item Chaplin, Melanie - VLA-WEYBRIDGE, UK
item Bartz, Jason - CREIGHTON UNIVERSITY
item Jenny, Allen - USDA, APHIS, NVSL
item Williams, Elizabeth - UNIVERSITY OF WYOMING

Submitted to: Meeting Abstract
Publication Acceptance Date: September 29, 2004
Publication Date: N/A
Technical Abstract: Raccoons (Procyon lotor) are omnivorous and their
diet may include carrion. It is, therefore, possible that in the wild
they may get exposed to carcasses of animals with transmissible
spongiform encephalopathies (TSEs). To determine the susceptibility of
raccoons to transmissible mink encephalopathy (TME), scrapie, and
chronic wasting disease (CWD), each of these agents was inoculated
intracerebrally into a group of 4 kits. Three uninoculated kits served
as controls. All raccoons in the TME-inoculated group developed
neurologic signs and were euthanized within 6 months post inoculation
(PI). In the scrapie-inoculated group, 3 animals became sick and were
euthanized between 18 - 22 PI. Although the fourth raccoon in this group
did not show any clinical signs, it was euthanized at 24 months PI. At
necropsy all clinically affected raccoons had extensive microscopic
lesions of spongiform encephalopathy and protease-resistant prion
protein (PrPres) was detected in the CNS by immunohistochemistry and
Western blot. In the CWD-inoculated group, 1 raccoon was euthanized at
39 months PI because of severe cystitis. Its brain was negative for
PrPres. At present, 5 years PI, the 3 remaining CWD-inoculated raccoons
are alive and apparently healthy. They will be kept under observation
for further period of 1 year (i.e 6 years PI) when the experiment will
be terminated. These preliminary findings demonstrate that TME and
scrapie can be transmitted to raccoons within 6 months and 2 years,
respectively, whereas CWD cannot. Based on these incubation periods, it
may be possible to differentiate these 3 TSEs. Such a laboratory model
would be relatively simple, fast and inexpensive for strain-typing of
unknown TSEs in the United States. Now that the relative susceptibiity
to IC transmission of each TSE has been established, oral transmission
studies of TSEs to raccoons are planned.

http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=167761tss





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