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From: TSS (
Subject: Comparative PRNP genotyping of U.S. cattle sires for potential association with BSE
Date: November 6, 2004 at 7:26 am PST

-------- Original Message --------
Subject: Comparative PRNP genotyping of U.S. cattle sires for potential association with BSE
Date: Sat, 06 Nov 2004 09:34:47 -0600
From: "Terry S. Singeltary Sr."

Comparative PRNP genotyping of U.S. cattle sires for potential
association with BSE

Christopher M. Seabury1, James E. Womack1, Jorge Piedrahita2 and
James N. Derr1 Contact Information

(1) Department of Veterinary Pathobiology, College of Veterinary
Medicine, Texas A&M University, College Station, Texas 77843-4467, USA

(2) Department of Molecular Biomedical Sciences, College of Veterinary
Medicine, North Carolina State University, Raliegh, North Carolina
27606, USA

Received: 14 April 2004 Accepted: 8 June 2004

Abstract The recent discovery of significant associations between
bovine spongiform encephalopathy (BSE) susceptibility in German cattle
and the frequency distributions of insertion/deletion (indel)
polymorphisms within the bovine PRNP gene prompted an evaluation of 132
commercial U.S. artificial insemination (AI) sires from 39 breeds.
Forward primer sequences from published primer sets targeting indels
within the putative bovine PRNP promoter, intron 1, and the 3prime UTR
(untranslated region) were synthesized with unique 5prime fluorescent
labels and utilized to develop a rapid multiplexed PCR assay for
identifying BSE-associated indels as well as facilitating polymorphism
analyses and/or marker-assisted selection. Significant differences (p <
0.05 all tests) were detected between the frequencies of bovine PRNP
promoter alleles for 48 healthy German cattle previously described and
132 commercial U.S. cattle sires. The frequency of the 23-bp promoter
allele observed for commercial U.S. cattle sires strongly resembled that
recently described for 43 BSE-affected German cattle. No significant
difference (pthinsp =thinsp 0.051) was detected between the
distributions of promoter genotypes for healthy German cattle and our
panel of commercial U.S. cattle sires. Interestingly, significant
differences (p < 0.01; p < 0.02) were also noted between the frequencies
and distributions of intron 1 alleles and genotypes, respectively, for
BSE-affected German cattle and our panel of U.S. cattle sires. No
significant allelic or genotypic differences were detected for the 14-bp
3prime UTR indel for any given comparison between German cattle and
commercial U.S. cattle sires.

Contact Information James N. Derr


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