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From: TSS (216-119-143-50.ipset23.wt.net)
Subject: Re: VERMONT SHEEP ATYPICAL SCRAPIE/TSE TESTING UPDATE
Date: June 14, 2004 at 9:18 am PST

In Reply to: Re: VERMONT SHEEP ATYPICAL SCRAPIE/TSE TESTING UPDATE posted by TSS on May 22, 2004 at 10:13 am:

-------- Original Message --------
Subject: Re: AW: [BSE-L] USDA did not test possible mad cows - Dr. Detwiler, what about those sheep?
Date: Sun, 13 Jun 2004 11:27:24 -0500
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@uni-karlsruhe.de
References: <13.2d20eaae.2df84fb9@aol.com> <40C8C7A0.1080107@wt.net>


######## Bovine Spongiform Encephalopathy #########

Greetings list members,

Thought I should let the list know that Dr. Detwiler kindly replied to my
question about the delayed 'atypical' TSE testing in the Vermont sheep and
tried to explain what caused the delay. If I interpreted it correctly,
seems it was the fault of the U.K. ;

-------- Original Message --------
Subject: Sheep
Date: Sat, 12 Jun 2004 14:26:04 EDT
From: LAVET22@aol.com
To: flounder@wt.net

Mr. Singeltary.

I hope this finds you well. As you are aware I left the USDA last
year. I can only update you on the sheep before that time. Contact was
established with the UK on doing the bioassay studies. They agreed.
However, we were prioritized after their own needs, hence the delay. I
am aware that there are now additional labs in Europe running the mouse
bioassay strain typing. You will have to contact USDA for further word.


Linda Detwiler
=========

My reply to Dr. Detwiler;


-------- Original Message --------
Subject: Re: Sheep
Date: Sat, 12 Jun 2004 13:53:57 -0500
From: "Terry S. Singeltary Sr."
To: LAVET22@aol.com
References: <54.2bd2ac1e.2dfca4bc@aol.com>

hello Dr. Detwiler,

thanks for your kind reply.

> However, we were prioritized after their own needs, hence the delay.


not sure i understand that?

> You will have to contact USDA for further word.


already done that, and there answer was;

>5/20/04
>

>Dear Mr. Singeltary,
>
>The Western blot tests on these animals were completed in April of this
>year. That means that we can begin the mouse inoculations. To get the
>results of the Western blot tests, you will need to submit a Freedom of
>Information Act request through our FOIA office. The FAX number there is
>301-734-5941.
>
>Have a nice day,
>
>Jim Rogers
>APHIS LPA
>

and with my previous attempts for information via the FOIA through
this administration (as you are probably very well aware of) they have
all been ignored/refused. so any further attempts would be fruitless i am
sure.

thanks anyway...

kindest regards,
Terry

LAVET22@aol.com wrote:

> Mr. Singeltary.

snip...

TSS

Terry S. Singeltary Sr. wrote:

> ######## Bovine Spongiform Encephalopathy
> #########
>
> Greetings Dr. Detwiler,
>
> glad to see you are still with us, you had become very silent lately.
> hope you are enjoying semi retirement.
>
> recently, i inquired through the BSE-L and via USDA official about
> those Vermont sheep via belgium which there was an Extraordinary
> Declaration of Emergency declared here in the USA due to
> atypical scrapie. The thread is;
>
> Confiscation of Sheep in Vermont and testing results ? Thu, 20 May 2004
> 12:10:03 -0500 "Terry S. Singeltary Sr." Bovine
> Spongiform Encephalopathy BSE-L
>
>
>
>> Imported
>> Belgium/Netherlands
>> Sheep Test Results
>> Background
>> Factsheet
>> Veterinary Services April 2002
>> APHIS
>
>
>
> snip...
>
>> Additional tests will be conducted to determine
>> exactly what TSE the animals haveBSE or scrapie.
>> These tests involve the use of bioassays that consist
>> of injecting mice with tissue from the infected animals
>> and waiting for them to develop disease. This testing
>> may take at least 2 to 3 years to complete.
>
>
>
> http://www.aphis.usda.gov/lpa/pubs/fsheet_faq_notice/fs_ahvtsheeptr.pdf
>
> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E.
> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
>
> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci
>
> d=fr20jy00-32
>
>
> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
>
> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&doci
>
> d=fr20jy00-31
>
>
> or if those old urls dont work, go here;
>
> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> - Terry S.
> Singeltary Sr. 7/20/00 (0)
>
> http://www.vegsource.com/talk/madcow/messages/7507.html
>
> I was told that ;
>
>
> -------- Original Message --------
> Subject: Re: hello Dr. Sutton...question please...scrapie...TSS
> Date: Thu, 20 May 2004 14:36:09 -0400
> From: Jim.D.Rogers@aphis.usda.gov
> To: flounder@wt.net
>
>
>
> Dear Mr. Singeltary,
>
> The Western blot tests on these animals were completed in April of this
> year. That means that we can begin the mouse inoculations. To get the
> results of the Western blot tests, you will need to submit a Freedom of
> Information Act request through our FOIA office. The FAX number there is
> 301-734-5941.
>
> Have a nice day,
>
> Jim Rogers
> APHIS LPA
> =========
>
>
> Dr. Detwiler, my question is, why have these very important test been
> delayed for so long when we were told they were to have been started
> some 2+ years ago?
>
> who made this call to delay these very important test and why ?
>
> thank you,
> with kindest regards,
>
> Terry
>
>
> Linda Detwiler wrote:
>
>> ######## Bovine Spongiform Encephalopathy
>> #########
>>
>> I m responding to Roland's post about my quote in the article by Steve
>> Mitchell. I spent a fair amount of time on the phone with Mr.
>> Mitchell on more than
>> one occasion. The quote was one aspect of our conversation. Even
>> the quote
>> included "probably". I explained about proper location and sampling
>> condition of the brain. I also added in our conversation that the
>> best methodology is
>> to utilize both a test for PrP as well as histopathology when examining
>> brains from cattle with CNS disease. This is why as early as
>> 1993-94 the USDA
>> began using IHC in its TSE testing regime at the National Veterinary
>> Services
>> Laboratory. However, utilizing only a PrP test eliminates the
>> possibility of
>> diagnosing another neurologic disease.
>>
>> All of the tests have advantages and disadvantages. For example, I
>> have been
>> to a number of laboratories in Europe and watched as technicians took
>> the
>> test samples from the brain stem. They sample hundreds per night.
>> If they get
>> distracted the may take the sample lateral, or rostral to the
>> obex. If this
>> animal was in an earlier stage of disease, there may be a very small
>> amount of
>> PrPsc and limited to a single location in the brain. If that sample
>> missed
>> the obex, the sample would be negative and life goes on. With any
>> test using
>> a homogenate I am not aware that there is any cross check for location.
>>
>> With IHC, the pathologist can determine location, however it too has
>> drawbacks in regard to sample condition. There is no perfect test.
>> There are
>> limitations to the tests themselves and there are limitations to all
>> of the aspects
>> of collection. Hence utilizing multiple tests especially for CNS
>> cases is
>> prudent. It is also prudent to examine other locations of the brain
>> in the event
>> a disease changes or something new emerges.
>>
>>
>> Linda Detwiler
>>
>> ######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>> ##########
>>
>>
>>
>
> ######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
> ##########
>

######### http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html ##########





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