To: BSE-L@uni-karlsruhe.de
######## Bovine Spongiform Encephalopathy #########
Greetings List Members,
i hear now that a 5th cow has gone done with CWD from the
studies of Amir Hamir et al. will Dr. Miller please
confirm or deny this please, and possibly explain why
this has not made the news, if in fact this is the case?
seems these cows infected with CWD/TSE did not display the
usual BSE symptoms. i wonder how many more are out there
in the field? course, we will never know unless someone
starts rapid TSE/BSE testing in sufficient numbers to find...
thank you,
kind regards,
terry
Date: Sat, 23 Nov 2002 18:54:49 -0600
Reply-To: BSE
Sender: BSE
From: "Terry S. Singeltary Sr."
Subject: CWD TO CATTLE by inoculation (ok, is it three or four???)
1: J Vet Diagn Invest 2001 Jan;13(1):91-6
Preliminary findings on the experimental transmission of chronic wasting
disease agent of mule deer to cattle.
Hamir AN, Cutlip RC, Miller JM, Williams ES, Stack MJ, Miller MW,
O'Rourke KI, Chaplin MJ.
National Animal Disease Center, ARS, USDA, Ames, IA 50010, USA.
To determine the transmissibility of chronic wasting disease (CWD) to
cattle and to provide information about clinical course, lesions, and
suitability of currently used diagnostic procedures for detection of CWD
in cattle, 13 calves were inoculated intracerebrally with brain
suspension from mule deer naturally affected with CWD. Between 24 and 27
months postinoculation, 3 animals became recumbent and were euthanized.
Gross necropsies revealed emaciation in 2 animals and a large pulmonary
abscess in the third. Brains were examined for protease-resistant prion
protein (PrP(res)) by immunohistochemistry and Western blotting and for
scrapie-associated fibrils (SAFs) by negative-stain electron microscopy.
Microscopic lesions in the brain were subtle in 2 animals and absent in
the third case. However, all 3 animals were positive for PrP(res) by
immunohistochemistry and Western blot, and SAFs were detected in 2 of
the animals. An uninoculated control animal euthanized during the same
period did not have PrP(res) in its brain. These are preliminary
observations from a currently in-progress experiment. Three years after
the CWD challenge, the 10 remaining inoculated cattle are alive and
apparently healthy. These preliminary findings demonstrate that
diagnostic techniques currently used for bovine spongiform
encephalopathy (BSE) surveillance would also detect CWD in cattle should
it occur naturally.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11243374&dopt=Abstract
Sat, Nov 23, 2002
Scientists unsure if CWD can jump species
By Jessica Bock Wausau Daily Herald jbock@wdhprint.com
snip...
Janice Miller, a veterinarian in charge of the experiment, said she
believes previous research shows it is hard for the disease to be
transmitted naturally from whitetail deer to dairy cattle. "Our study
says nothing of how it could be transmitted in natural surroundings,"
she said.
Miller has been studying the transmission of CWD from mule deer to
cattle since 1997. Since then, chronic wasting disease was transmitted
to four out of 13 cattle injected with brain tissue from naturally
infected mule deer, she said.
In Wyoming, Williams has been studying cattle that were given a
concoction of diseased brain tissue orally, and five years into the
study the animals remain healthy, Miller said. No one knows if chronic
wasting disease could ever spread to another species through natural
surroundings.
"Our experience is that it's pretty hard to predict," Miller said.
http://www.wausaudailyherald.com/wdhlocal/277564794712612.shtml
greetings list,
> Since then, chronic wasting disease was
> transmitted to four out of 13 cattle
is this a typo by the media or has another cow gone down with CWD since
the preliminary findings were found?
TSS
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