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From: TSS ()
Subject: Biochemical identification of bovine spongiform encephalopathies in cattle
Date: October 14, 2007 at 11:03 am PST

Biochemical identification of bovine spongiform encephalopathies in cattle

Jean-Noël Arsac1, Anne-Gaëlle Biacabe1, Julia Nicollo1, Anna Bencsik1 and
Thierry Baron1

(1) Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité
ATNC, 31 Avenue Tony Garnier, 69364 Lyon Cedex 07, France

Thierry Baron

Received: 18 April 2007 Revised: 22 June 2007 Accepted: 12 July 2007
Published online: 1 August 2007

Abstract Important changes have occurred in the post-mortem diagnosis of
bovine spongiform encephalopathy (BSE) in recent years. We have evaluated a
commercially available Western blot method (TeSeE® Wb) as a potential means
of confirming BSE. This method was (i) highly sensitive, compared with a
biochemical confirmatory Western blot method (AFSSA-Wb) previously used in
France and (ii) more sensitive than two routinely used highly sensitive
rapid tests (TeSeE® ELISA, HerdCheck® BSE). We show that this high
sensitivity is mainly due to the antibody used (Sha31). Interestingly,
TeSeE® Wb was also able to diagnose the two currently recognised deviant BSE
phenotypes (H-type and L-type or BASE). The initially described molecular
features of these atypical forms of BSE were also readily recognised,
although sensitivity of the method may be differently affected by the chosen
Ab compared with typical BSE. This method is thus of potential interest for
future evaluations of BSE confirmatory methods.


Finally, although the BSE epidemic is now decreasing in most affected
countries and should be controlled within the next few years [1, 14, 42,
43], the recent identification of “unusual” BSE cases [7, 15, 33] has raised
some concern that other forms of prion diseases, possibly with a different
etiology, might affect cattle. Whereas this is supported by a number of
recent experimental studies, which suggest that at least two TSE strains
exist in cattle that differ from the one initially isolated during the BSE
epidemic in the UK, [2, 6, 8, 13], the question of possible “sporadic” cases
of BSE has been raised [3, 11]. In such a situation, these cases would be
detected and would still potentially represent some health concern even
after the end of the current BSE food-borne epidemic, and any diagnostic
methods should be able to detect such “unusual” cases in the future.
Interestingly, our series of BSE samples contained one or two samples of
each of the deviant BSE phenotypes, which have been described so far, and we
were able to determine the features of each case. Not only were such cases
readily confirmed by the TeSeE Western blot method, but the unusual features
initially described in such cases were also recognised whatever the
positivity level of the sample. Interestingly, an unusual PrPres pattern is
specifically observed in H-type BSE with C-terminal antibodies such as SAF84
which was previously shown to be the result of the presence of an
additional, more C-terminally cleaved and either glycosylated or not, PrPres
form [8]; this was accompanied with an increased sensitivity of such
antibodies for this particular BSE type, illustrating potential
strain-specific differences between the performances of diagnostic methods.

All together, our data describe the features of a highly sensitive and
readily available biochemical confirmatory approach for BSE diagnosis that
could help in the future assessment of confirmatory methods.

Acknowledgments The authors are grateful to J-M. Bilheude for his technical
support and helpful discussions. We also wish to thank M. Lavoine for her
excellent technical assistance. This work was partly supported by NEUROPRION
European network of excellence (EUROSTRAINS project).

see full text ;

I find it odd to say the least that no mention of the fact that h-BASE data strongly support the link, or at least a common
ancestry, between a sCJD subtype, as stated below. why is this $ i suppose the fact that h-BASE in the USA, and the
continued increase of sporadic cjd of 'UNKNOWN STRAIN' is of no importance to anyone. pretty handy though, out of sight, out of mind. ...TSS

BASE Transmitted to Primates and MV2 sCJD Subtype Share PrP27-30 and PrPSc
C-terminal Truncated Fragments

We here show that the PrPSc pattern obtained in infected primates is identical to BASE
and sCJD MV-2 subtype. These data strongly support the link, or at least a common
ancestry, between a sCJD subtype and BASE.

This work was supported by Neuroprion (FOOD-CT-2004-506579)

Transmission of Italian BSE and BASE Isolates in Cattle Results into a
Typical BSE Phenotype and a Muscle Wasting Disease

This study further confirms that BASE is caused by a distinct prion isolate and discloses a novel disease
phenotype in cattle, closely resembling the phenotype previous reported in scrapie-inoculated
cattle and in some subtypes of inherited and sporadic Creutzfeldt-Jakob disease.


Date: Mon, 24 Sep 2007 21:31:55 -0500

I suggest that you all read the data out about h-BASE and sporadic CJD, GSS,
blood, and some of the other abstracts from the PRION2007. ...


USA BASE CASE, (ATYPICAL BSE), AND OR TSE (whatever they are calling it
today), please note that both the ALABAMA COW, AND THE TEXAS COW, both were
''H-TYPE'', personal communication Detwiler et al Wednesday, August 22, 2007
11:52 PM. ...TSS

PLEASE NOTE IN USA CJD UPDATE AS AT JUNE 2008, please note steady increase

1 Acquired in the United Kingdom; 2 Acquired in Saudi Arabia; 3 Includes 17 inconclusive and 9 pending (1 from 2006, 8
from 2007); 4 Includes 17 non-vCJD type unknown (2 from 1996, 2 from 1997, 1 from 2001, 1 from 2003, 4 from 2004, 3 from 2005, 4 from 2006) and 36 type pending (2 from 2005, 8 from 2006, *** 26 from 2007)

still disgusted in cloudy Baycliff, Texas .........flounder

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