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From: TSS ()
Subject: Rapid testing leads to the underestimation of the scrapie prevalence in an affected sheep and goat flock
Date: July 28, 2007 at 8:45 am PST

Rapid testing leads to the underestimation of the scrapie prevalence in an affected sheep and goat flock

Claudia Reckzeha, b, Christine Hoffmanna, Anne Buschmanna, Silke Budac, Klaus-Dieter Budrasc, Karl-Friedrich Recklingd, Steffi Bellmannd, Hartmut Knobloche, Georg Erhardtf, Reinhard Friesb and Martin H. Groschupa, ,
aFriedrich-Loeffler-Institut, Institute for Novel and Emerging Diseases, Insel Riems, Germany
bInstitute of Meat Hygiene and Technology, Faculty of Veterinary Medicine, Freie Universität Berlin, Germany
cInstitute of Veterinary Anatomy, Freie Universität Berlin, Germany
dLandesuntersuchungsamt für Gesundheits-, Umwelt- und Verbraucherschutz, Stendal, Germany
eVeterinär- und Lebensmittelüberwachungsamt des Landkreises Anhalt-Zerbst, Germany
fDepartment of Animal Breeding and Genetics, Justus-Liebig University of Giessen, Germany

Available online 8 April 2007.

Abstract

To obtain a more detailed understanding of the prevalence of classical scrapie infections in a heavily affected German sheep flock (composed of 603 sheep and 6 goats), we analysed 169 sheep and 6 goats that carried the genotypes susceptible to the disease and that were therefore culled following discovery of the index case. The initial tests were performed using the Biorad TeSeE ELISA and reactive results were verified by official confirmatory methods (OIE-immunoblot and/or immunohistochemistry (IHC)) to demonstrate the deposition of scrapie-associated PrPSc in the brain stem (obex). This approach led to the discovery of 40 additional subclinically scrapie-infected sheep. Furthermore, peripheral lymphatic and nervous tissue samples of the 129 sheep and 6 goats with a negative CNS result were examined by IHC in order to identify any preclinical infections which had not already spread to the central nervous system (CNS). Using this approach we found 13 additional sheep with PrPSc depositions in the gut-associated lymph nodes (GALT) as well as in the enteric nervous system. Moreover, in most of these cases PrPSc was also deposited in the spleen and in the retropharyngeal and superficial cervical lymph nodes. Taken together, these results show a 30.3% infection prevalence in this scrapie-affected flock. Almost 7.4% of the infected animals harboured PrPSc exclusively in the peripheral lymphatic and nervous tissue and were therefore missed by the currently used testing strategy.

Keywords: Preclinical scrapie; Immunohistochemistry; Lymphoid tissue

Corresponding author at: Institute for Novel and Emerging Infectious Diseases at the Friedrich-Loeffler-Institut, Boddenblick 5a, 17493 Greifswald, Germany. Tel.: +49 38351 7163; fax: +49 38351 7191.


http://www.sciencedirect.com/


Veterinary Microbiology
Volume 123, Issue 4, 31 August 2007, Pages 320-327
Recent Progress in Prion Research - Scientific Advances Reported at the Concluding Meeting of the German TSE Research Platform, German TSE Research Platform


http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%235190%232007%23998769995%23663856%23FLA%23&_cdi=5190&_pubType=J&view=c&_auth=y&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=3654394c66bb4b913491733a9341463c


TSS



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