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From: TSS ()
Subject: Detection of Bovine Meat and Bone Meal in Animal Feed at a Level of 0.1%
Date: December 17, 2006 at 1:08 pm PST

Detection of Bovine Meat and Bone Meal in Animal Feed at a Level of 0.1%

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Author(s): Henk J.M. Aarts 1, | El M. Bouw 2, | Jaap B. Buntjer 3, | Johannes A. Lenstra 4, | Leo W.D. van Raamsdonk 5


Print ISSN: 1060-3271
Volume: 89 | Issue: 6
Cover date: November/December 2006
Page(s): 1443-1446

Abstract text


For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBRGreen fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected.

Author(s): Henk J.M. Aarts 1, | El M. Bouw 2, | Jaap B. Buntjer 3, | Johannes A. Lenstra 4, | Leo W.D. van Raamsdonk 5


Author(s) affiliations

1RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands.
henk.aarts@wur.nl
2RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands.
3Keygene N.V., PO Box 216, 6700 AE Wageningen, The Netherlands.
4Utrecht University, Faculty of Veterinary Medicine, Yalelaan 8, 3584 CM Utrecht, The Netherlands.
5RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands.

http://www.atypon-link.com/AOAC/doi/abs/10.5555/jaoi.89.6.1443


as little as 1 mg (or 0.001 gm) caused 7% (1 of 14) of the cows to come down with BSE;


Risk of oral infection with bovine spongiform encephalopathy agent in primates

Corinne Ida Lasmézas, Emmanuel Comoy, Stephen Hawkins, Christian Herzog, Franck Mouthon, Timm Konold, Frédéric Auvré, Evelyne Correia, Nathalie Lescoutra-Etchegaray, Nicole Salès, Gerald Wells, Paul Brown, Jean-Philippe Deslys
Summary The uncertain extent of human exposure to bovine spongiform encephalopathy (BSE)--which can lead to variant Creutzfeldt-Jakob disease (vCJD)--is compounded by incomplete knowledge about the efficiency of oral infection and the magnitude of any bovine-to-human biological barrier to transmission. We therefore investigated oral transmission of BSE to non-human primates. We gave two macaques a 5 g oral dose of brain homogenate from a BSE-infected cow. One macaque developed vCJD-like neurological disease 60 months after exposure, whereas the other remained free of disease at 76 months. On the basis of these findings and data from other studies, we made a preliminary estimate of the food exposure risk for man, which provides additional assurance that existing public health measures can prevent transmission of BSE to man.


snip...


BSE bovine brain inoculum

100 g 10 g 5 g 1 g 100 mg 10 mg 1 mg 0·1 mg 0·01 mg

Primate (oral route)* 1/2 (50%)

Cattle (oral route)* 10/10 (100%) 7/9 (78%) 7/10 (70%) 3/15 (20%) 1/15 (7%) 1/15 (7%)

RIII mice (ic ip route)* 17/18 (94%) 15/17 (88%) 1/14 (7%)

PrPres biochemical detection

The comparison is made on the basis of calibration of the bovine inoculum used in our study with primates against a bovine brain inoculum with a similar PrPres concentration that was

inoculated into mice and cattle.8 *Data are number of animals positive/number of animals surviving at the time of clinical onset of disease in the first positive animal (%). The accuracy of

bioassays is generally judged to be about plus or minus 1 log. ic ip=intracerebral and intraperitoneal.

Table 1: Comparison of transmission rates in primates and cattle infected orally with similar BSE brain inocula


Published online January 27, 2005

http://www.thelancet.com/journal/journal.isa


[Docket No. 03-025IFA] FSIS Prohibition of the Use of Specified Risk
Materials for Human Food and Requirement for the Disposition of
Non-Ambulatory Disabled Cattle

http://www.fsis.usda.gov/OPPDE/Comments/03-025IFA/03-025IFA-2.pdf


[Docket No. FSIS-2006-0011] FSIS Harvard Risk Assessment of Bovine
Spongiform Encephalopathy (BSE)


http://www.fsis.usda.gov/OPPDE/Comments/2006-0011/2006-0011-1.pdf

THE SEVEN SCIENTIST REPORT ***


http://www.fda.gov/ohrms/dockets/dockets/02n0273/02n-0273-EC244-Attach-1.pdf


PAUL BROWN M.D.

http://www.fda.gov/ohrms/dockets/dockets/02n0273/02n-0273-c000490-vol40.pdf


9 December 2005
Division of Dockets Management (RFA-305)

SEROLOGICALS CORPORATION
James J. Kramer, Ph.D.
Vice President, Corporate Operations

http://www.fda.gov/ohrms/dockets/dockets/02n0273/02n-0273-c000383-01-vol35.pdf

Embassy of Japan

http://www.fda.gov/ohrms/dockets/dockets/02n0273/02N-0273-EC240.htm


TSS



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