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From: TSS ()
(1) Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy Received: 16 December 2005 Accepted: 2 March 2006 Published online: 20 April 2006 Summary. The pathogenesis of natural scrapie in Sarda breed sheep was investigated in 1050 asymptomatic and 49 sick sheep from scrapie-affected flocks. Central and peripheral nervous system, along with lymphoreticular system (LRS) tissues, were subjected to immunohistochemistry (IHC) and Western-blotting (WB) for detection of pathological isoform of the prion protein (PrPSc). A total of 69 of the 1050 clinically healthy sheep were found to be infected with scrapie, with PrPSc being detected in both the central nervous system (CNS) and enteric nervous system (ENS) plexuses of 60 of the sheep, while IHC and WB yielded evidence of (PrPSc) deposition only in lymphoid tissues of the remaining 9 clinically healthy sheep. PrPSc was also detected in the CNS, as well as in ENS plexuses from all of the 49 clinically affected sheep. Nevertheless, 18 of the 69 clinically healthy animals (26%, 17 ARQ/ARQ and 1 ARQ/AHQ sheep), along with 3 ARQ/ARQ sheep (6%) of the clinically affected group, showed no IHC or WB evidence of PrPSc in lymphoid tissues, but PrPSc could be still detected in their CNS and ENS plexuses. The study demonstrates dual CNS and ENS PrPSc deposition in Sarda sheep with scrapie, in spite of an apparent lack of lymphoid tissue involvement in a number of cases. -------------------------------------------------------------------------------- TSS
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