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From: TSS ()
Subject: Re: Report of the first oral inoculation of BSE prion UPDATE
Date: January 4, 2006 at 9:39 am PST

In Reply to: Report of the first oral inoculation of BSE prion UPDATE posted by TSS on January 4, 2006 at 7:00 am:

The development of the intracerebral inoculation
method and BSE experimental transmissions to
calves

Shigeo Fukuda, Satoshi Nikaido, Yosh!taka Matsui, Soichi Kageyama and
Sadao Onoe

Hokkaido Animal Research Center, 5-39 Shintoku-nishi, Shintoku-cho,
Kamikawa-gun 081-0038 Japan s.fukuda@agri.pref.hokkaido.jp

Abstract

It is very difficult to obtain cattle infected with bovine spongiform en-
cephalopathy (BSE) because there is no means of diagnosing BSE in live
animals. This has been the obstacle of the BSE research. It is pressing
need to make sure of enough cattle and bovine tissues infected with BSE
for it. An intracerebral (i.e.) inoculation is the most efficient route of a
prion disease transmission in each animal. However there are few reports
about methods of i.e. inoculation to cattle. We developed the efficient
and safe method of an i.e. inoculation to calves. In addition, we chal-
lenged BSE brain tissues to calves. It is the first BSE experimental
transmission to cattle in Japan.

The inoculation site was decided using heads of carcass calves. A
frontal bone was drilled by a pin-drill with a diameter of 2 mm on the spot
of 1 cm nose side from the front edge of the bulge between horns and 2 cm
right side from the median line. The tip of the disposable needle (70 mm,
18G) could penetrate a midbrain of calves. Four calves were injected in-
tracerebrally with stained normal brain tissue homogenates to this site un-
der mollification and local anesthesia. Clinical symptoms of these calves
were observed for 3 or 4 hours and the distribution of the inoculated brain
homogenate were investigated on the postmortem.

There were no calves presenting obvious neurological symptoms after
challenges. The almost of all homogenate spread ventricles and cerebral
aqueduct. Cerebrospinal fluid flowed over from the hole of inoculation
site by intracranial pressure, which leads to the leakage of infectious fac-
tors and the secondary infection for calves. Using a 3 x 8 mm stainless
screw has settled this problem.

183

PRIONS
Food and Drug Safety
Springer
T. Kitamoto (Ed.)

TSS



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