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From: TSS ()
Subject: Abnormal PrP in the retina of the most commonly subtype sCJD
Date: August 20, 2005 at 6:48 am PST

Abnormal PrP in the retina of the most commonly subtype sCJD
Fri Aug 19, 2005 22:00
68.238.104.92


SCIENTIFIC REPORT

Abnormal prion protein in the retina of the most commonly occurring subtype of sporadic Creutzfeldt-Jakob disease
M W Head1, A H Peden1, H M Yull1, D L Ritchie1, R E Bonshek2, A B Tullo2 and J W Ironside1
1 National CJD Surveillance Unit, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, UK
2 Academic Department of Ophthalmology, Manchester Royal Eye Hospital, Manchester M13 9WH, UK


Correspondence to:
Dr M W Head
National CJD Surveillance Unit, Bryan Matthews Building, Western General Hospital, Edinburgh EH4 2XU, UK; m.w.head@ed.ac.uk

ABSTRACT
Background: Involvement of the eye has been reported in patients with variant Creutzfeldt-Jakob disease (vCJD), but there is disagreement on whether retinal involvement occurs in sporadic Creutzfeldt-Jakob disease (sCJD).

Methods: Western blotting, paraffin embedded tissue blotting, and immunohistochemistry were used to test whether the abnormal form of the prion protein (PrPSc) accumulates to detectable levels in the eye in a case of the most common subtype of sCJD (MM1).

Results: Low levels of PrPSc were detectable in the retina, localised to the plexiform layers of the central retina. PrPSc was not detectable in other ocular tissues.

Conclusions: The abnormal form of the prion protein is present in the retina in the most common sCJD subtype (MM1), albeit at levels lower than those found previously in vCJD and in sCJD of the VV2 subtype.

--------------------------------------------------------------------------------

Abbreviations: sCJD, sporadic Creutzfeldt-Jakob disease; vCJD, variant Creutzfeldt-Jakob disease


Keywords: Creutzfeldt-Jakob disease; prion protein; PRNP codon 129 genotype; retina

http://bjo.bmjjournals.com/cgi/content/abstract/89/9/1131?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=prion&searchid=1124503447145_1536&stored_search=&FIRSTINDEX=0&volume=89&issue=9&journalcode=bjophthalmol

Eye procedure raises CJD concerns


By Steve Mitchell
Medical Correspondent


Washington, DC, Nov. 18 (UPI) -- A New York man who died from a rare brain disorder similar to mad cow disease in May underwent an eye procedure prior to his death that raises concerns about the possibility of transmitting the fatal disease to others, United Press International has learned.

The development comes on the heels of the announcement Thursday by U.S. Department of Agriculture officials of a possible second case of mad cow disease in U.S. herds.

Richard Da Silva, 58, of Orange County, N.Y., died from Creutzfeldt Jakob disease, an incurable brain-wasting illness that strikes about one person per million.

Richard's wife Ann Marie Da Silva told UPI he underwent a check for the eye disease glaucoma in 2003, approximately a year before his death. The procedure involves the use of a tonometer, which contacts the cornea -- an eye tissue that can contain prions, the infectious agent thought to cause CJD.

Ann Marie's concern is that others who had the tonometer used on them could have gotten infected.

A 2003 study by British researchers suggests her concerns may be justified. A team led by J.W. Ironside from the National Creutzfeldt-Jakob Disease Surveillance Unit at the University of Edinburgh examined tonometer heads and found they can retain cornea tissue that could infect other people -- even after cleaning and decontaminating the instrument.

"Retained corneal epithelial cells, following the standard decontamination routine of tonometer prisms, may represent potential prion infectivity," the researchers wrote in the British Journal of Ophthalmology last year. "Once the infectious agent is on the cornea, it could theoretically infect the brain."

Prions, misfolded proteins thought to be the cause of mad cow, CJD and similar diseases, are notoriously difficult to destroy and are capable of withstanding most sterilization procedures.

Laura Manuelidis, an expert on these diseases and section chief of surgery in the neuropathology department at Yale University, agreed with the British researchers that tonometers represent a potential risk of passing CJD to other people.

Manuelidis told UPI she has been voicing her concern about the risks of corneas since 1977 when her own study, published in the New England Journal of Medicine, showed the eye tissue, if infected, could transmit CJD.

At the time the procedure was done on Richard Da Silva, about a year before he died, she said it was "absolutely" possible he was infectious.

The CJD Incidents Panel, a body of experts set up by the U.K. Department of Health, noted in a 2001 report that procedures involving the cornea are considered medium risk for transmitting CJD. The first two patients who have a contaminated eye instrument used on them have the highest risk of contracting the disease, the panel said.

In 1999, the U.K. Department of Health banned opticians from reusing equipment that came in contact with patients' eyes out of concern it could result in the transmission of variant CJD, the form of the disease humans can contract from consuming infected beef products.

Richard Da Silva was associated with a cluster of five other cases of CJD in southern New York that raised concerns about vCJD.

None of the cases have been determined to stem from mad cow disease, but concerns about the cattle illness in the United States could increase in light of the USDA announcement Thursday that a cow tested positive on initial tests for the disease. If confirmed, this would be the second U.S. case of the illness; the first was detected in a Washington cow last December. The USDA said the suspect animal disclosed Thursday did not enter the food chain. The USDA did not release further details about the cow, but said results from further lab tests to confirm the initial tests were expected within seven days.

Ann Marie Da Silva said she informed the New York Health Department and later the eye doctor who performed the procedure about her husband's illness and her concerns about the risk of transmitting CJD via the tonometer.

The optometrist -- whom she declined to name because she did not want to jeopardize his career -- "didn't even know what this disease was," she said.

"He said the health department never called him and I called them (the health department) back and they didn't seem concerned about it," she added. "I just kept getting angrier and angrier when I felt I was being dismissed."

She said the state health department "seems to have an attitude of don't ask, don't tell" about CJD.

"There's a stigma attached to it," she said. "Is it because they're so afraid the public will panic? I don't know, but I don't think that the answer is to push things under the rug."

New York State Department of Health spokeswoman Claire Pospisil told UPI she would look into whether the agency was concerned about the possibility of transmitting CJD via tonometers, but she had not called back prior to story publication.

Disposable tonometers are readily available and could avoid the risk of transmitting the disease, Ironside and colleagues noted in their study. Ann Marie Da Silva said she asked the optometrist whether he used disposable tonometers and "he said 'No, it's a reusable one.'"

Ironside's team also noted other ophthalmic instruments come into contact with the cornea and could represent a source of infection as they are either difficult to decontaminate or cannot withstand the harsh procedures necessary to inactivate prions. These include corneal burrs, diagnostic and therapeutic contact lenses and other coated lenses.

Terry Singletary, whose mother died from a type of CJD called Heidenhain Variant, told UPI health officials were not doing enough to prevent people from being infected by contaminated medical equipment.

"They've got to start taking this disease seriously and they simply aren't doing it," said Singletary, who is a member of CJD Watch and CJD Voice -- advocacy groups for CJD patients and their families.

U.S. Centers for Disease Control and Prevention spokeswoman Christine Pearson did not return a phone call from UPI seeking comment. The agency's Web site states the eye is one of three tissues, along with the brain and spinal cord, that are considered to have "high infectivity."

The Web site said more than 250 people worldwide have contracted CJD through contaminated surgical instruments and tissue transplants. This includes as many as four who were infected by corneal grafts. The agency noted no such cases have been reported since 1976, when sterilization procedures were instituted in healthcare facilities.

Ironside and colleagues noted in their study, however, many disinfection procedures used on optical instruments, such as tonometers, fail. They wrote their finding of cornea tissue on tonometers indicates that "no current cleaning and disinfection strategy is fully effective."

Singletary said CDC's assertion that no CJD cases from infected equipment or tissues have been detected since 1976 is misleading.

"They have absolutely no idea" whether any cases have occurred in this manner, he said, because CJD cases often aren't investigated and the agency has not required physicians nationwide report all cases of CJD.

"There's no national surveillance unit for CJD in the United States; people are dying who aren't autopsied, the CDC has no way of knowing" whether people have been infected via infected equipment or tissues, he said.

Ann Marie Da Silva said she has contacted several members of her state's congressional delegation about her concerns, including Rep. Sue Kelly, R-N.Y., and Sen. Charles Schumer, D-N.Y.

"Basically, what I want is to be a positive force in this, but I also want more of a dialogue going on with the public and the health department," she said.

http://washingtontimes.com/upi-breaking/20041118-030642-2974r.htm

Cadaver corneal transplants -- without family permission

Houston, Texas channel 11 news 28 Nov 99
Reported by Terry S. Singeltary Sr.son of CJD victim

http://www.mad-cow.org/~tom/dec99_news.html#bbb

CJD and intraocular surgery

Ophthalmic surgery and Creutzfeldt-
Jakob disease

http://www.vegsource.com/talk/madcow/messages/92788.html

-------- Original Message --------
Subject: Ophthalmic surgery and Creutzfeldt-Jakob disease
Date: Fri, 19 Mar 2004 09:39:43 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: BSE-L@uni-karlsruhe.de


######## Bovine Spongiform Encephalopathy #########

April 2004; Vol. 88, No. 4

URL: http://www.bjophthalmol.com/content/vol88/issue4/index.shtml?etoc


Series editor: David Taylor
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . .
Ophthalmic surgery and Creutzfeldt-
Jakob disease
P S-Juan, H J T Ward, R De Silva, R S G Knight, R G Will
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . .
Although the evidence does not suggest that contaminated
ophthalmic instruments represent a risk of onward transmission of
sporadic CJD, this conclusion should be treated with caution
The occurrence of variant
Creutzfeldt-Jakob disease (vCJD)
and the probable causal link with
bovine spongiform encephalopathy
(BSE) in cattle have increased interest
in the search for possible environmental
sources of sporadic CJD (sCJD).
Presumed iatrogenic CJD is rare. Up to
the year 2000 there had been 267 cases
reported worldwide: three cases secondary
to human corneal grafting (one
confirmed, one probable, and one possible
case), 114 related to human dura
mater grafts, 139 related to human
growth hormone treatment, four related
to human pituitary gonadotrophin therapy,
and seven linked to neurosurgical
procedures or stereotactic EEG electrodes.
1 Because of the marked resistance
of the infectious agent of CJD to
conventional sterilisation techniques,
there is concern about the possibility of
transmission of infection via surgical
instruments in contact with infected
tissue, especially in neurosurgery or
ophthalmic surgery.

SNIP...TO LONG TO POST HERE, PLEASE VIEW FULL TEXT HERE;


http://www.vegsource.com/talk/madcow/messages/92120.html


a bit of history;

The Eyes have it/CJD * and they could be stealing them from YOUR loved
one, hence the
spread of CJD (aka MADCOW DISEASE) will spread...

1
http://www.vegsource.com/talk/lyman/messages/7242.html

2
http://www.vegsource.com/talk/lyman/messages/7643.html

3
http://www.vegsource.com/talk/lyman/messages/7708.html

http://jama.ama-assn.org/issues/v282n23/full/jlt1215-5.html

http://mad-cow.org/~tom/dec99_news.html#bbb

Testimony of Bess Believeaux, Lions Eye Bank of Central Texas
(Submission to the Jan. 18/19 meeting of the
TSE Advisory Committee)

http://www.fda.gov/ohrms/dockets/ac/01/slides/3681s2_16.pdf

TSS Submission to the same Committee;
http://www.fda.gov/ohrms/dockets/ac/01/slides/3681s2_09.pdf

Tissue Banks International (TBI), Gerald J Cole
http://www.fda.gov/ohrms/dockets/ac/01/slides/3681s2_13.pdf

re-use contact lenses
http://www.vegsource.com/talk/lyman/messages/7691.html

TSS

########### http://mailhost.rz.uni-karlsruhe.de/warc/bse-l.html ############


Cadaver corneal transplants -- without family permission

http://www.mad-cow.org/~tom/dec99_news.html#bbb

TSS

#################### https://lists.aegee.org/bse-l.html ####################




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